2′ Modified oligonucleotides
First Claim
Patent Images
1. An oligonucleotide having the formula:
-
or a salt thereof, wherein;
each B independently is a protected or unprotected purine or pyrimidine base in the β
-anomeric configuration;
W3 and W4 are each independently H, PO3−
2, a protecting group, or an intermediate moiety in the formation of an internucleotide link which when reacted with the appropriate —
OH results in Z;
n is an integer of 1-200;
each Z is independently is P(O)O, P(O)S or P(O)N(R)2 provided at least one Z is not P(O)O;
each R is independently H or alkyl (C1-6);
each A is OCH3, H, OH, O—
Pr wherein Pr is a protecting group, or X—
Y wherein;
X is O; and
Y is substituted or unsubstituted alkyl (C2-20) or substituted or unsubstituted alkenyl (C2-20), wherein the substituents are a halogen, OH, NH2, SH, —
O—
, —
CH2CONH2, —
CH2COOCH2CH3, or CH2CONHCH2NH2; and
at least one A is OCH3.
1 Assignment
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Accused Products
Abstract
Oligomers which have substituents on the 2′ position are resistant to oligonucleases and furthermore can be derivatized to deliver reagents or drugs, to carry label, or to provide other properties.
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Citations
6 Claims
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1. An oligonucleotide having the formula:
-
or a salt thereof, wherein; each B independently is a protected or unprotected purine or pyrimidine base in the β
-anomeric configuration;
W3 and W4 are each independently H, PO3−
2, a protecting group, or an intermediate moiety in the formation of an internucleotide link which when reacted with the appropriate —
OH results in Z;
n is an integer of 1-200;
each Z is independently is P(O)O, P(O)S or P(O)N(R)2 provided at least one Z is not P(O)O;
each R is independently H or alkyl (C1-6);
each A is OCH3, H, OH, O—
Pr wherein Pr is a protecting group, or X—
Y wherein;
X is O; and
Y is substituted or unsubstituted alkyl (C2-20) or substituted or unsubstituted alkenyl (C2-20), wherein the substituents are a halogen, OH, NH2, SH, —
O—
, —
CH2CONH2, —
CH2COOCH2CH3, or CH2CONHCH2NH2; and
at least one A is OCH3.- View Dependent Claims (2, 3, 4, 5, 6)
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Specification