Electrodes linked via conductive oligomers to nucleic acids
First Claim
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1. A method of detecting a target nucleic acid sequence in a nucleic acid sample comprising:
- a) hybridizing a probe nucleic acid to said target sequence, to form a hybridization complex, wherein said probe nucleic acid is covalently attached to a first electron transfer moiety comprising an electrode, wherein said hybridization complex comprises a second electron transfer moiety, and wherein said electrode further comprises a monolayer; and
, b) detecting electron transfer between said electrode and said second electron transfer moiety, as an indicator of the presence or absence of said target sequence.
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Abstract
The invention relates to nucleic acids covalently coupled to electrodes via conductive oligomers. More particularly, the invention is directed to the site-selective modification of nucleic acids with electron transfer moieties and electrodes to produce a new class of biomaterials, and to methods of making and using them.
144 Citations
14 Claims
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1. A method of detecting a target nucleic acid sequence in a nucleic acid sample comprising:
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a) hybridizing a probe nucleic acid to said target sequence, to form a hybridization complex, wherein said probe nucleic acid is covalently attached to a first electron transfer moiety comprising an electrode, wherein said hybridization complex comprises a second electron transfer moiety, and wherein said electrode further comprises a monolayer; and
,b) detecting electron transfer between said electrode and said second electron transfer moiety, as an indicator of the presence or absence of said target sequence. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14)
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Specification
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Current AssigneeRoche Molecular Systems (Roche Holding AG)
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Original AssigneeClinical Micro Sensors Inc. (Roche Holding AG)
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InventorsKayyem, Jon F., Gozin, Michael, Yu, Changjun, OʼConnor, Stephen D.
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Primary Examiner(s)Marschel, Ardin H.
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Application NumberUS09/577,429Time in Patent Office904 DaysField of Search435/6, 436/501, 422/68.1US Class Current435/6.11CPC Class CodesC07H 21/00 Compounds containing two or...C12Q 1/6825 Nucleic acid detection invo...
