Spray tip for a microfluidic laboratory microchip

• US 6,481,648 B1
• Filed: 05/15/2000
• Issued: 11/19/2002
• Est. Priority Date: 10/01/1999
• Status: Expired due to Term

• Current Assignee: Agilent Technologies, Inc.
• Original Assignee: Agilent Technologies, Inc.
• Inventors: Zimmermann, Hans-Peter
• Primary Examiner(s): EVANS, ROBIN OCTAVIA
• Application Number:
  US09/570,947
• Time in Patent Office: 918 Days
• Field of Search: 239/589, 239/690, 239/691, 239/692
• US Class Current: 239/690
• CPC Class Codes: B01L 3/0241 Drop counters; Drop formers making arrays for combinatorial libraries B01J19/0046; automation of dispensing for analysis G01N35/10
• CPC Class Codes: G01N 27/44717 Arrangements for investigating the separated zones, e.g. localising zones
• CPC Class Codes: H01J 49/04 Arrangements for introducing or extracting samples to be analysed, e.g. vacuum locks; Arrangements for external adjustment of electron- or ion-optical components

Abstract

A laboratory microchip includes a carrier with a micro spray tip projecting vertically out of a plane of the carrier. The carrier and spray tip are designed as a monolithic or single unit. The spray tip includes a passage channel which conveys the substances to be sprayed from a channel to a spray tip aperture. The channel is a part of a channel structure which is enclosed to the outside by a cover plate. The spray tip includes, on the sides of the spray aperture, an area which tapers conically inwards. Connected to this tapered area is a constant cross-section drill hole forming an annular internal step formation at the transition between the two areas. The outer diameter of the conically tapering tip is locally reduced in proximity to the aperture to provide a finer tip to the spray tip. The finer tip increases the electrical fields occurring at the tip, to further increase the forces which act on the substances and for dispersion spraying.

Claims

1. Microfluidic chip for the chemical, physical, and/or biological analysis or synthesis of substances, comprising a channel structure arranged on one side of a carrier having an essentially flat portion, the substances being capable of being moved according to the channel structure under the imposition of an electrical potential, an integrated micro spray tip for dispersion spraying of the substances, the micro spray tip and the carrier being a monolithic single unit;

the micro spray tip and the channel structure being arranged on opposite sides of the same carrier, the micro spray tip including a passage channel projecting substantially at right angles to a plane of the channel structure of the flat portion of the carrier;

the micro spray tip, on the side of the carrier including the channel structure being connected by the passage channel thereof to the channel structure of the carrier flat portion in a substance-conveying manner; and

an electrical voltage source connected to the micro spray tip in an electrically-conductive manner for dispersion spraying of the substance.

2. The microfluidic microchip of claim 1 further including a liquid channel connected to the channel structure of the carrier flat portion and the passage channel, the liquid channel being connected to the voltage source and arranged so there is hydrodynamic flow in the passage channel and there are electro-osmotic flow and hydrodynamic flow in the liquid channel, the hydrodynamic flow being at least equal to the electro-osmotic flow.

3. Microfluidic microchip according to claim 1, wherein the micro spray tip and the carrier are made of a polymer material.

4. Microfluidic microchip of claim 3 wherein the polymer is selected from the group consisting essentially of PEEK (polyether etherketone), PI (polyimide), and PMMA (polymethyl metacrylate).

5. Microfluidic microchip according to claim 1, wherein the micro spray tip is connected to a separation channel on the carrier for conveying the chemical, physical, and/or biological analysis or synthesis of the substances.

6. Microfluidic microchip according to claim 1, wherein the micro spray tip is arranged in an outer area of the surface plane of the carrier.

7. Microfluidic microchip according to claim 1 wherein the microchip includes, on the side of the carrier including the channel structure, a cover plate enclosing the channel structure downwards, to provide a substance seal.

8. Microfluidic microchip according to claim 1 wherein the outer diameter of the micro spray tip is between about 10 and 100 μ

m, and the height of the micro spray tip is about 1 mm.

9. Microfluidic microchip according to claim 1, wherein the voltage source has a potential for causing matched movement of the substances to one another in such a way that an electro-osmotic substance flow directed towards the micro spray tip is equal to a hydraulic substance flow of the substance towards the spray tip.

10. Microfluidic microchip according to claim 9, further including a source for adjusting the hydraulic substance flow rate between the micro spray tip and the volume outside the microchip.

11. Microfluidic microchip according to claim 10 wherein the source for adjusting includes a variable voltage source.

12. Microfluidic microchip according to claim 10 wherein the source for adjusting includes a variable differential pressure source.

13. Microfluidic microchip according to claim 1, further including an electricity supply for the micro spray tip, the electricity supply including a liquid junction resulting from the substances flowing to the micro spray tip.

14. Microfluidic microchip according to claim 9, wherein a liquid junction where the voltage source is connected to the substances is arranged so a slight liquid overpressure pertains in relation to the pressure prevailing at the micro spray tip.

15. Microfluidic microchip according to claim 14, wherein the liquid junction is arranged so that a liquid overpressure enables an additional liquid to be mixed with the substance being sprayed to the outside volume.

16. The microfluidic microchip of claim 1 wherein the microchip is adapted for use with substances having sufficient electrical conductivity to provide an electrical path through the substances from the voltage source to an exit region of the spray tip, the spray tip being arranged to enable the voltage source to be connected to the exit region only by the substances.

17. The microfluidic microchip of claim 16 wherein walls of the spray tip adapted to contact the substances are electrical insulators.

18. The microfluidic microchip of claim 17 further including a liquid channel connected to the channel structure of the carrier flat portion and the passage channel, the liquid channel being connected to the voltage source and arranged so there is hydrodynamic flow in the passage channel and there are electro-osmotic flow and hydrodynamic flow in the liquid channel, the hydrodynamic flow being at least equal to the electro-osmotic flow.

19. The microfluidic microchip of claim 16 further including a liquid channel connected to the channel structure of the carrier flat portion and the passage channel, the liquid channel being connected to the voltage source and arranged so there is hydrodynamic flow in the passage channel and there are electro-osmotic flow and hydrodynamic flow in the liquid channel, the hydrodynamic flow being at least equal to the electro-osmotic flow.

20. Microfluidic chip for the chemical, physical, and/or biological analysis or synthesis of substances, comprising a channel structure arranged on one side of a carrier having an essentially flat portion, the substances being capable of being moved according to the channel structure under the imposition of an electrical potential, an integrated micro spray tip for dispersion spraying of the substances, the micro spray tip including a passage channel projecting substantially at right angles to a plane of the channel structure of the flat portion of the carrier;

the micro spray tip on the side of the carrier including the channel structure being connected by the passage channel thereof to the channel structure of the carrier flat portion in a substance-conveying manner; and

an electrical voltage source connected to the micro spray tip in an electrically-conductive manner for dispersion spraying of the substance, the microchip being adapted for use with substances having sufficient electrical conductivity to provide an electrical path through the substances from the voltage source to an exit region of the spray tip, the spray tip being arranged to enable the voltage source to be connected to the exit region only by the substances.

21. The microfluidic microchip of claim 20 wherein walls of the spray tip adapted to contact the substances are electrical insulators.

22. The microfluidic microchip of claim 21 further including a liquid channel connected to the channel structure of the carrier flat portion and the passage channel, the liquid channel being connected to the voltage source and arranged so there is hydrodynamic flow in the passage channel and there are electro-osmotic flow and hydrodynamic flow in the liquid channel, the hydrodynamic flow being at least equal to the electro-osmotic flow.

23. The microfluidic microchip of claim 20 further including a liquid channel connected to the channel structure of the carrier flat portion and the passage channel, the liquid channel being connected to the voltage source and arranged so there is hydrodynamic flow in the passage channel and there are electro-osmotic flow and hydrodynamic flow in the liquid channel, the hydrodynamic flow being at least equal to the electro-osmotic flow.