Meso- and microfluidic continuous flow and stopped flow electroösmotic mixer

• US 6,482,306 B1
• Filed: 09/22/1999
• Issued: 11/19/2002
• Est. Priority Date: 09/22/1998
• Status: Expired due to Fees

• Current Assignee: University of Washington
• Original Assignee: University of Washington
• Inventors: Macounova, Katerina, Cabrera, Catherine, Yager, Paul, Kamholz, Andrew, Holl, Mark R.
• Primary Examiner(s): Warden, Sr., Robert J.
• Assistant Examiner(s): Olsen, Kaj K.
• Application Number:
  US09/404,454
• Time in Patent Office: 1,154 Days
• Field of Search: 204/450, 204/454, 204/600, 204/601, 366/341, 366/349, 137/808, 137/825, 137/827
• US Class Current: 204/600
• CPC Class Codes: B01F 33/05 Mixers using radiation, e.g. magnetic fields or microwaves to mix the material B01F23/2133, B01F23/238, B01F23/55, B01F33/3031, B01F33/3032 take precedence
• CPC Class Codes: B01F 33/052 the energy being electric fields for electrostatically charging of the ingredients or compositions for mixing them
• CPC Class Codes: B01F 33/3031 using electro-hydrodynamic [EHD] or electro-kinetic [EKI] phenomena to mix or move the fluids
• CPC Class Codes: B01F 33/3032 using magneto-hydrodynamic [MHD] phenomena to mix or move the fluids
• CPC Class Codes: B01L 3/5027 by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip B01L3/5023 takes precedence; micromixers B01F33/30; microreactors for synthesis B01J19/0093; microcapillary devices in general B81B1/00
• CPC Class Codes: Y10T 137/2087 Means to cause rotational flow of fluid [e.g., vortex generator]
• CPC Class Codes: Y10T 137/2191 By non-fluid energy field affecting input [e.g., transducer]

Abstract

An electroösmotic mixing device and a method for mixing one or more fluids for use in meso- or microfluidic device applications. The mixing device provides batch or continuous mixing of one or more fluids in meso- or microfluidic channels. An electric field is generated in the channel in substantial contact with chargeable surfaces therein. No alterations of the geometry of existing flow paths need be made, and the degree of mixing in the device can be controlled by the length of the electrodes, the flow rate past the electrodes, and the voltage applied to those electrodes. The degree of mixing is affected by choice of materials for the chargeable surface (in some cases by the selection of materials or coatings for channel walls) and the ionic strength of the fluids and the type and concentration of ions in the fluids. The ionic strength of fluids to be mixed is sufficiently low to allow electroosmotic flow. The method and device of this invention is preferably applied to fluids to having low ionic strength less than or equal to about 1 mM.

Claims

1. A microfluidic channel comprising an electroosmotic mixing region therein which comprises:

a) an interior surface;

b) first and second ends defining a flow axis, z, of said channel;

c) closures positioned at said first and second ends;

d) two or more electrodes;

i. that are on said interior surface;

ii. in which chargeable surfaces are between them on said interior surface;

iii. which are positioned at a common z axis position along said channel in said mixing region;

or such that two electrodes form said closures; and

e) means for applying a voltage across two or more of said electrodes to cause electroosmotic mixing.

2. The microfluidic channel of claim 1 wherein the electrode gap is between about 10 μ

m and about 1 mm.

3. The microfluidic channel of claim 1 wherein the gap between the chargeable surfaces is between about 1 to about 10 times the gap between the electrodes.

4. The microfluidic channel of claim 1 wherein said applied voltage is from 0 to about 1.5 v.

5. The microfluidic channel of claim 1 wherein the chargeable surfaces are glass.

6. The microfluidic channel of claim 1 wherein the electrodes are copper or gold.

7. The microfluidic channel of claim 1 wherein the chargeable surfaces are substantially perpendicular to the surfaces of the electrodes.

8. The microfluldic channel of claim 1 wherein one or more of said electrodes is a coating or deposit on said interior surface.

9. The microfluidic channel of claim 1 wherein one or more of said electrodes is a wire.

10. The microfluidic channel of claim 1 wherein one or more of the electrodes is a grid.

11. A microfluidic channel comprising an electroosmotic mixing region which comprises:

a) an interior surface;

b) first and second ends defining a flow axis Z, of said channel,

c) two or more electrodes comprising one or more gold or copper coatings on said interior surface, wherein said electrodes;

i. are positioned at a common z axis position along said channel;

ii. are separated from each other by a distance of about 10 μ

m to about 1 mm, have two or more chargeable surfaces between them, and have a cross-channel separation distance, d; and

d) means for applying a voltage across two or more of said electrodes to cause electroosmotic mixing; and

e) at least two channel closures positioned upstream and downstream of said electrodes;

wherein said chargeable surfaces are glass, are substantially perpendicular to the surfaces of said electrodes, and have a cross-channel separation distance w such that w/d is about 1 to about 10; and

wherein said applied voltage is from 0 to about 1.5V.

12. A microfluidic channel comprising an electroosmotic mixing region which comprises:

a) an interior surface;

b) first and second ends defining a flow axis Z, of said channel;

c) two or more electrodes comprising one or more gold or copper coatings on said interior surface, and which;

i. form one or more closures that are positioned at said first and second ends of said mixing region;

ii. are separated from each other by a distance of about 10 μ

m to about 1 mm, have two or more chargeable surfaces between them, and have a cross-channel separation distance, d;

d) means for applying a voltage across two or more of said electrodes to cause electroosmotic mixing; and

e) at least two channel closures positioned upstream and downstream of said electrodes;

wherein said chargeable surfaces are glass, are substantially perpendicular to the surfaces of said electrodes, and have a cross-channel separation distance w such that w/d is 1 to about 10; and

wherein said applied voltage is from 0 to about 1.5V.