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Method for polynucleotide amplification

  • US 6,482,590 B1
  • Filed: 11/06/1997
  • Issued: 11/19/2002
  • Est. Priority Date: 12/20/1996
  • Status: Expired due to Fees
First Claim
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1. A method for selectively extending an oligonucleotide primer along a specific target polynucleotide sequence in a mixture of polynucleotides, said method comprising:

  • (a) providing in combination said mixture and a modified oligonucleotide having a 3′

    -end that is inefficiently extendable in a template dependent manner along any polynucleotide sequence; and

    (b) controlling the template dependent extension of said oligonucleotide primer along said specific target polynucleotide sequence by controlling the degradation of said 3′

    -end of said modified oligonucleotide thereby producing said oligonucleotide primer.

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