Microscope, in particular a stereomicroscope, and a method of superimposing two images

• US 6,483,948 B1
• Filed: 09/29/1997
• Issued: 11/19/2002
• Est. Priority Date: 12/23/1994
• Status: Expired due to Term

• Current Assignee: Leica Instruments Singapore Pte Ltd (Danaher Corporation)
• Original Assignee: Leica AG
• Inventors: Braunecker, Bernhard, Spink, Roger
• Primary Examiner(s): ROGERS, SCOTT A
• Application Number:
  US08/849,707
• Time in Patent Office: 1,877 Days
• Field of Search: 382/255, 382/254, 382/275, 382/131, 382/128, 382/154, 348/25, 348/42, 348/46, 348/47, 348/54, 348/80, 348/79, 356/904, 359/376, 359/377, 359/378, 359/466, 359/470, 359/462, 359/368, 359/369, 345/424, 345/632, 345/633, 600/417, 600/429, 600/407, 606/130, 250/201.3
• US Class Current: 382/255
• CPC Class Codes: G02B 21/22 Stereoscopic arrangements

Abstract

The invention concerns an imaging system comprising a first device (82) for acquiring image data for an object (93) using light, in particular a microscope, and a second device (94) for acquiring and displaying image data (in particular, of the kind not immediately visible to the eye) for the object (93) using (in particular invisible) radiation, preferably electromagnetic wave or particle radiation such as X-rays (X-ray or CT photographs), MRI or positron radiation. The system is also provided with a superimposition device (95) for superimposing the two sets of image data to form a single image which can be turned towards an observer. The optical parameters of the first device (82), e.g. the microscope and its image distortions, are incorporated in the image to be superimposed, thereby providing the observer with a dimensionally and geometrically accurate display of the two superimposed images.

Claims

1. In an image representing device used with a microscope for detecting visible pictorial information concerning an object with visible light and a secondary apparatus for detecting invisible pictorial information concerning said object which is initially hidden from a viewer'"'"'s eye, said image representing device having means for superimposing said visible pictorial information and said invisible pictorial information into a single image, the improvement comprising:

adaptive control means for detecting change in three-dimensional geometric imaging parameters of said microscope occurring as a result of adjustment of said microscope and for using said detected change to correct said invisible pictorial information to coincide geometrically with said visible pictorial information, wherein said geometric imaging parameters include primary imaging parameters comprising microscope alignment, viewing angle, and magnification of the microscope light path, and secondary imaging parameters comprising visual field and depth of focus.

2. The improvement according to claim 1, wherein said geometric imaging parameters further include tertiary imaging parameters comprising aberration of the metric and distortion.

3. The improvement according to claim 1, wherein said microscope includes a range finder for determining a z-distance to said object and a magnification measuring device, and said adaptive control means is responsive to parameters determined by said range finder and said magnification measuring device.

4. The improvement according to claim 3, wherein said adaptive control means includes memory means for storing predetermined correction information for use in correcting said invisible pictorial information.

5. The improvement according to claim 2, wherein said adaptive control means includes memory means for storing predetermined correction information for use in correcting said invisible pictorial information.

6. The improvement according to claim 4, wherein said correction information includes correction algorithms which are type-specific to said microscope light path.

7. The improvement according to claim 5, wherein said correction information includes correction algorithms which are type-specific to said microscope light path.

8. The improvement according to claim 7, wherein said correction information includes a plurality of computational formulas which are series-specific to said microscope, said computational formulas being derived from a prior calibration of said adaptive control means with said microscope through a range of microscope adjustments.

9. The improvement according to claim 8, wherein a standardized object of known geometry is observed during said prior calibration, and said standardized object is uniformly detectable for rays of said microscope and said secondary apparatus.

10. The improvement according to claim 3, wherein said adaptive control means includes a data preparation unit for calculating adaptation parameters for proportionally correct scaling of said invisible pictorial information to coincide with said visible pictorial information.

11. A process for superimposing initially different first and second image data, said first image data coming from a first apparatus and said second image data coming from a second apparatus, comprising the steps of:

(A) providing a standardized object having a known geometry described by actual dimensional data;

(B) converting said actual dimensional data into a reference voxel volume representative of said known geometry and storing said reference voxel volume in memory;

(C) placing said standardized object before said first apparatus and detecting calibration image data of said standardized object from a plurality of different perspectives;

(D) converting said calibration image data of said standardized object into a calibration voxel volume representative of said standardized object as observed through said first apparatus subject to primary, secondary, and tertiary imaging parameters of said first apparatus;

(E) comparing said calibration voxel volume with said reference voxel volume to derive a first computational formula based on differences between said reference and calibration voxel volumes, and storing said first computational formula;

(F) modifying said second image data using said first computational formula to improve congruence between said first and second image data;

(G) superimposing said first and second image data on one another; and

(H) presenting said superimposed image data to a viewer.

12. The process according to claim 11, further including the steps of:

(A) placing said standardized object before said second apparatus and detecting additional calibration image data of said standardized object from a plurality of different perspectives;

(B) converting said additional calibration image data of said standardized object into an additional calibration voxel volume representative of said standardized object as observed through said second apparatus subject to secondary and tertiary imaging parameters of said second apparatus;

(C) comparing said additional calibration voxel volume with said reference voxel volume to derive a second computational formula based on differences between said reference and additional calibration voxel volumes, and storing said second computational formula; and

(D) modifying said first image data using said second computational formula to further improve congruence between said first and second image data.

13. The process according to claim 11, wherein said first apparatus is continuously adjusted and said second image data is continuously modified using said first computational formula as said first apparatus is adjusted.

14. The process according to claim 11, further including the step of superimposing adjustment data describing the adjustment status of said first apparatus on said superimposed image data presented to said viewer.