Site specific ligation of proteins to synthetic particles

• US 6,485,718 B1
• Filed: 04/11/2000
• Issued: 11/26/2002
• Est. Priority Date: 04/13/1999
• Status: Expired due to Fees

• Current Assignee: Pharmacia Corporation (Pfizer Inc.)
• Original Assignee: Pharmacia Corporation (Pfizer Inc.)
• Inventors: Lee, Stephen C., Parthasarathy, Rajani V.
• Primary Examiner(s): Riley, Jezia
• Application Number:
  US09/547,008
• Time in Patent Office: 959 Days
• Field of Search: 530/300, 530/350, 424/78.17
• US Class Current: 424/78.17
• CPC Class Codes: A61K 47/595 Polyamides, e.g. nylon polyamino acids A61K47/62
• CPC Class Codes: B82Y 5/00 Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
• CPC Class Codes: C07K 1/1077 by covalent attachment of residues other than amino acids or peptide residues, e.g. sugars, polyols, fatty acids

Abstract

The present invention relates to site-specific conjugation of synthetic particles to proteins.

Claims

1. A method for site specifically attaching a synthetic particle to the N-terminus of a protein, said method comprising the steps of:

a) converting a polypeptide containing a serine as its N-terminal amino acid to an aldehyde;

b) converting the amine of a synthetic particle to a oxiamine; and

c) combining said aldehyde polypeptide containing a serine as its N-terminal amino acid to said synthetic particle at said oxiamine.