Systems and methods for performing reactions in an unsealed environment

• US 6,485,913 B1
• Filed: 10/02/2000
• Issued: 11/26/2002
• Est. Priority Date: 03/10/1999
• Status: Expired due to Term

• Current Assignee: Agena Bioscience Inc. (Mesa Laboratories Inc.)
• Original Assignee: Sequenom Incorporated (Laboratory Corporation Of America Holdings)
• Inventors: Becker, Thomas, Cantor, Charles, Köster, Hubert
• Primary Examiner(s): Jones, W. Gary
• Assistant Examiner(s): Taylor, Janell E.
• Application Number:
  US09/678,620
• Time in Patent Office: 785 Days
• Field of Search: 435/6, 436/177, 159/1, 159/47.1, 422/64, 422/65, 422/66, 422/67
• US Class Current: 506/13
• CPC Class Codes: B01J 19/16 Preventing evaporation or oxidation of non-metallic liquids by applying a floating layer, e.g. of microballoons in storage tanks B65D90/42
• CPC Class Codes: B01J 2219/00317 Microwell devices, i.e. having large numbers of wells
• CPC Class Codes: B01J 2219/00367 capillary
• CPC Class Codes: B01J 2219/00378 Piezoelectric or ink jet dispensers
• CPC Class Codes: B01J 2219/00495 Means for heating or cooling the reaction vessels
• CPC Class Codes: B01J 2219/00603 Making arrays on substantially continuous surfaces
• CPC Class Codes: B01J 2219/0068 Means for controlling the apparatus of the process
• CPC Class Codes: C40B 60/14 for creating libraries
• CPC Class Codes: G01N 2035/00287 movable lid/cover for sample or reaction tubes
• CPC Class Codes: Y10T 436/11 Automated chemical analysis
• CPC Class Codes: Y10T 436/25375 Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]

Abstract

Open systems for performing submicroliter reactions are provided. The systems can include a support for performing the reaction; a liquid dispensing system for dispensing a submicroliter amount of a liquid to a site on or in the support; a temperature controlling device for regulating the temperature of the support; and an interface for controlling the amount of liquid dispensed from the liquid dispensing system are provided. Methods using the systems are also provided.

Claims

1. A system for performing a reaction, comprising:

a support for performing the reaction;

a nanoliter dispensing pipette for dispensing a submicroliter amount of a liquid onto the support;

a temperature controlling device for regulating the temperature of the support; and

means for controlling the amount of liquid dispensed, wherein;

the amount of liquid dispensed corresponds to the amount of liquid that evaporates from the support; and

the controlling means comprises software that calculates the amount of liquid that evaporates and signals the dispensing pipette to deliver an amount of liquid that corresponds to the amount that evaporates, wherein the system is open.

2. The open system of claim 1, further comprising a means for determining the temperature of a liquid on the support.

3. The open system of claim 1, wherein the support comprises a bead, pin, comb, wafer, well or microchip.

4. The open system of claim 1, wherein the support is capable of linking to a biopolymer or to a biological particle.

5. The open system of claim 1, further comprising a detection system for monitoring the reaction.

6. The system of claim 1, wherein the support comprises a surface for containing a submicroliter reaction volume that comprises an array of hydrophilic regions and adjacent hydrophobic regions.

7. The system of claim 1, further comprising a support having a target site for retaining or containing a reaction mixture.

8. A system, comprising:

a solid support having a target site for retaining or containing submicroliter quantities of a liquid;

a liquid dispensing system for dispensing a liquid to the target site;

a temperature controlling system, which regulates the temperature of the solid support; and

an interface for indicating an amount of liquid to be dispensed to the target site, wherein the amount of liquid to be dispensed corresponds to an amount of liquid that evaporates from the target site.

9. The system of claim 8, wherein the amount of liquid to be dispensed is determined based on the temperature of the liquid on the solid support.

10. The system of claim 8, wherein the interface monitors the level of a liquid on the target site.

11. The system of claim 10, wherein the interface comprises an electrical circuit comprising the liquid on the target site.

12. The system of claim 8, wherein the temperature controlling system comprises a Peltier element.

13. The system of claim 8, further comprising a detection system.

14. A system, comprising:

a solid support having a target site, which can contain a submicroliter amount of a liquid;

a liquid dispensing system, which can dispense a liquid to the target site;

a temperature controlling system, which regulates the temperature of the solid support; and

means for regulating an amount of liquid dispensed from the liquid dispensing system, wherein the amount of liquid dispensed corresponds to an amount of liquid that evaporates from the target site, wherein the system is open.

15. The system of claim 14, wherein the regulating means comprises an interface in communication with the target site.

16. The system of claim 14, wherein the regulating means comprises manual input.