Reagent test strip for analyte determination having hemolyzing agent
First Claim
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1. A reagent test strip for use in determining analyte concentration in a physiological sample, said strip comprising:
- a porous matrix;
at least one member of an analyte oxidation signal producing system; and
at least one hemolyzing agent, wherein said hemolyzing agent is present in an amount sufficient to produce hemolysis which is equivalent to hemolysate concentration in a physiological sample applied to said porous matrix in an amount ranging from about 5 to 20% by volume.
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Abstract
Reagent test strips and methods for their use in the determination of the concentration of an analyte, e.g. glucose, in a physiological sample, are provided. The subject reagent test strips include one or more members of an analyte oxidation signal producing system and at least one hemolyzing agent. The subject reagent test strips and methods are particularly suited for use in the detection of blood glucose concentrations. Also provided are kits that include the subject test strips for use in practicing the subject methods.
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Citations
16 Claims
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1. A reagent test strip for use in determining analyte concentration in a physiological sample, said strip comprising:
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a porous matrix;
at least one member of an analyte oxidation signal producing system; and
at least one hemolyzing agent, wherein said hemolyzing agent is present in an amount sufficient to produce hemolysis which is equivalent to hemolysate concentration in a physiological sample applied to said porous matrix in an amount ranging from about 5 to 20% by volume. - View Dependent Claims (2, 3, 4, 5)
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6. A reagent test strip for use in determining blood glucose concentration, said test strip comprising:
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a porous matrix;
a glucose oxidation based signal producing system; and
at least one hemolyzing agent, wherein said hemolyzing agent is present in an amount sufficient to produce hemolysis which is equivalent to hemolysate concentration in a physiological sample applied to said porous matrix in an amount ranging from about 5 to 20% by volume. - View Dependent Claims (7, 8, 9, 10)
a glucose oxidizing enzyme;
a peroxidase; and
an indicator that is converted to a chromogenic product in the presence of hydrogen peroxide and said peroxidase.
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8. The test strip according to claim 7, wherein said indicator comprises a dye couple.
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9. The test strip according to claim 6, wherein said test strip comprises an amount of hemolyzing agent sufficient to produce hemolysis which is equivalent to hemolysate concentration in a physiological sample applied to said porous matrix in an amount ranging from about 10 to 20% by volume.
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10. The test strip according to claim 6, wherein said glucose oxidizing enzyme is glucose oxidase.
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11. A reagent test strip for use in determining blood glucose concentration, said test
strip comprising: -
(a) a porous matrix;
(b) a glucose oxidation based signal producing system comprising;
(i) a glucose oxidase;
(ii) a peroxidase; and
(iii) an indicator that is converted to a chromogenic product in the presence of hydrogen peroxide and said peroxidase; and
(c) at least one hemolyzing agent in an amount sufficient to produce hemolysis which is equivalent to hemolysate concentration in a physiological sample applied to said porous matrix in an amount ranging from about 5 to 20% by volume. - View Dependent Claims (12, 13, 14, 15, 16)
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Specification