Electrodes having a continuous, crystalline metal nitride coating and method of use

• US 6,485,961 B1
• Filed: 07/18/2000
• Issued: 11/26/2002
• Est. Priority Date: 12/05/1996
• Status: Expired due to Term

• Current Assignee: Maxcyte, Inc. (Entremed, Incorporated)
• Original Assignee: Maxcyte, Inc. (Entremed, Incorporated)
• Inventors: Meserol, Peter
• Primary Examiner(s): Beisner, William H.
• Application Number:
  US09/618,654
• Time in Patent Office: 861 Days
• Field of Search: 435/173.6, 435/285.2, 422/44, 204/600, 204/606, 204/290.01, 204/290.12, 204/290.13, 204/290.09, 204/450, 204/547
• US Class Current: 435/285.2
• CPC Class Codes: A61N 1/0412 Specially adapted for transcutaneous electroporation, e.g. including drug reservoirs
• CPC Class Codes: A61N 1/042 Material of the electrode
• CPC Class Codes: A61N 1/0424 Shape of the electrode
• CPC Class Codes: A61N 1/327 for enhancing the absorption properties of tissue, e.g. by electroporation
• CPC Class Codes: C12M 35/02 Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
• CPC Class Codes: C12N 13/00 Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves

Abstract

An improved electrode for use in generating an electrical field in a saline solution is provided. In particular, a continuous crystalline metal nitride coated electrode is provided for use in a variety of saline solution applications, such as in an electrophoresis device for separating proteins or nucleic acids or an electroporation apparatus for the encapsulation of biologically-active substances in various cell populations. A method and apparatus are provided for the encapsulation of biologically-active substances in red blood cells, characterized by an optionally automated, continuous-flow, self-contained electroporation system which allows withdrawal of blood from a patient, separation of red blood cells, encapsulation of a biologically-active substances in the cells, and optional recombination of blood plasma and the modified red blood cells thereby producing blood with modified biological characteristics.

Claims

1. Apparatus for electrical stimulation of particles in a saline solution, comprising:

walls defining a particle electrical stimulation chamber; and

a pair of electrodes disposed along opposing walls of said chamber, said electrodes comprising means for placing said electrodes in electrical communication with a source of electrical energy, whereby particles in said chamber are subjected to an electrical field;

said electrodes each further comprising an external surface with at least a portion thereof corresponding to the emission of the electrical field having a continuous crystalline metal nitride coating.

2. The apparatus of claim 1, wherein the electrical energy is high voltage, pulsed electrical energy.

3. The apparatus of claim 1, wherein at least a portion the surface of both electrodes corresponding to the emission of the electrical field has a continuous crystalline metal nitride coating.

4. The apparatus of claim 1, wherein the continuous crystalline metal nitride coating is selected from the group consisting of titanium nitride, titanium aluminum nitride, chromium nitride, and zirconium nitride.

5. A flow electroporation device comprising:

a housing having internal walls defining a continuous internal chamber through said housing, said internal chamber being configured to receive a continuous flow volume of blood therethrough;

a first electrode having a first specified length and disposed within said internal chamber;

a second electrode having a second specified length and disposed within said internal chamber in spaced-apart relation to said first electrode;

said first and second electrodes being arranged within said internal chamber to permit said continuous flow volume of blood flowing through said internal chamber to pass between said electrodes;

said first and second electrodes being operative when connected to a pulse generator to emit pulses of electronic energy from said first electrode through the blood to the second electrode as said continuous flow volume of blood flows through said internal chamber; and

at least a portion of one of said first and second electrodes having a continuous crystalline metal nitride coating;

the frequency of pulses of electronic energy emitted through said electrodes, said first and second specified lengths of said electrodes, and the rate of flow of said continuous flow volume of blood flowing through said internal chamber being such that blood cells in said volume of blood may be electroporated while flowing through said internal chamber.

6. The apparatus of claim 5, wherein the electrical energy is high voltage, pulsed electrical energy.

7. The apparatus of claim 5, wherein at least a portion the surface of both electrodes corresponding to the emission of the electrical field has a continuous crystalline metal nitride coating.

8. The apparatus of claim 5, wherein the continuous crystalline metal nitride coating is selected from the group consisting of titanium nitride, titanium aluminum nitride, chromium nitride, and zirconium nitride.

9. A method of generating an electronic signal in a saline solution comprising:

a. providing a device comprising walls defining a chamber for pair of electrodes disposed along opposing sides of said chamber, said electrodes comprising means for placing said electrodes in electrical communication with a source of electrical energy, whereby the saline solution is subjected to an electrical field;

said electrodes each further comprising an external surface with at least a portion thereof corresponding to emission of the electrical field having a continuous crystalline metal nitride coating;

b. adding a saline solution to the chamber; and

,

c. generating an electronic signal in the saline solution.

10. The method of claim 9, wherein the saline solution contains a biological particle.

11. The method of claim 10, wherein the biological particle is a cell.

12. The method of claim 9, wherein the electrical signal is high voltage, pulsed electrical energy.

13. The method of claim 9, wherein at least a portion the surface of both electrodes corresponding to the emission of the electrical field has a continuous crystalline metal nitride coating.

14. The method of claim 9, wherein the continuous metal nitride coating is selected from the group consisting of titanium nitride, titanium aluminum nitride, chromium nitride, and zirconium nitride.

15. An improved electrode comprising an electrode having continuous crystalline metal nitride coating on at least one surface thereof, wherein the coating inhibits the migration of metal ions from the electrode.

16. The electrode of claim 15, wherein at least a surface portion of the electrode corresponding to the emission of an electrical field has a continuous crystalline metal nitride coating.

17. The electrode of claim 15, wherein the continuous crystalline metal nitride coating is selected from the group consisting of titanium nitride, titanium aluminum nitride, chromium nitride, and zirconium nitride.