Growth stimulation of biological cells and tissue by electromagnetic fields and uses thereof

• US 6,485,963 B1
• Filed: 06/02/2000
• Issued: 11/26/2002
• Est. Priority Date: 06/02/2000
• Status: Expired due to Term

• Current Assignee: National Aeronautics and Space Administration US Government As Represented By The Administrator of The, United States Of America As Represented By The Administrator Of The National Aeronautics And Space Administration
• Original Assignee: United States Of America As Represented By The Administrator Of The National Aeronautics And Space Administration
• Inventors: Goodwin, Thomas J., Wolf, David A.
• Primary Examiner(s): Redding, David A.
• Application Number:
  US09/587,028
• Time in Patent Office: 907 Days
• Field of Search: 435/173.1, 435/173.8, 435/298.2, 435/299.1
• US Class Current: 435/298.2
• CPC Class Codes: C12M 25/14 Scaffolds; Matrices in general C12N5/0068
• CPC Class Codes: C12M 35/02 Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
• CPC Class Codes: C12N 13/00 Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves

Abstract

The present invention provides systems for growing two or three dimensional mammalian cells within a culture medium facilitated by an electromagnetic field, and preferably, a time varying electromagnetic field. The cells and culture medium are contained within a fixed or rotating culture vessel, and the electromagnetic field is emitted from at least one electrode. In one embodiment, the electrode is spaced from the vessel. The invention further provides methods to promote neural tissue regeneration by means of culturing the neural cells in the claimed system. In one embodiment, neuronal cells are grown within longitudinally extending tissue strands extending axially along and within electrodes comprising electrically conductive channels or guides through which a time varying electrical current is conducted, the conductive channels being positioned within a culture medium.

Claims

1. A system for growing three-dimensional biological cells, comprising:

a rotating wall vessel containing a cell-rich medium;

cell carriers placed within said vessel; and

an electrical current passed through said cell carriers for producing an electromagnetic field within said cell-rich medium.

2. The system of claim 1, wherein said cell carriers are selected from the group consisting of spherical disks and bioscaffold matrices containing multiple parallel channels.

3. The system of claim 2, wherein said channels are coated with a bioattractive material.

4. The system of claim 3, wherein said channels, coated with said bioattractive material, have longitudinal axes, and wherein said electrical current flows through said channels along said longitudinal axes.

5. The system of claim 1, wherein said bioattractive material is selected from the group consisting of titanium, zirconium and platinum.

6. The system of claim 1, wherein said biological cells comprise mammalian cells selected from the group consisting of neuronal cells, normal human neuronal progenitor cells and cells responding to waveform.

7. The system of claim 1, wherein said rotating wall vessel is selected from the group consisting of a rotating wall perfused vessel and a rotating wall batch-fed vessel.

8. The system of claim 1, wherein said electrical current induces a cellular response at the gene level.

9. The system of claim 8, wherein said cellular response is cellular control of growth and differentiation at the gene level.

10. The system of claim 1, wherein said electrical current induces cellular control of growth and differentiation, for suppressing or enhancing growth regulatory functions at gene level.

11. The system of claim 10, wherein said gene is associated with embryogenesis.

12. The system of claim 1, wherein said electrical current is associated with a time varying potential.

13. The system of claim 12, wherein said time varying electrical potential is in the form of a square wave.

14. A method of culturing biological cells in the system of claim 1, comprising the steps of:

Inoculating said cells into said vessel;

rotating said vessel to initiate the attachment of said cells to cell carriers;

applying an electrical potential to said cell carriers; and

measuring the growth of said cells.

15. The method of claim 14, wherein said vessel is rotated at a speed of from about 10 RPM to about 30 RPM.

16. The method of claim 15, wherein said electrical potential is associated with a current applied at a strength range of from about 1 mA to about 6 mA.

17. The method of claim 14, wherein said electrical potential is a time varying potential.

18. The method of claim 17, wherein said time varying potential is in a square wave.