Method for sex determination of mammalian offspring

• US 6,489,092 B1
• Filed: 08/21/2000
• Issued: 12/03/2002
• Est. Priority Date: 07/01/1997
• Status: Expired due to Fees

• Current Assignee: Vicam LP (Waters Corporation)
• Original Assignee: Vicam LP (Waters Corporation)
• Inventors: Livingston, David, George, Susan, Basker, Christopher J., Benjamin, Thomas L., Kohn, Barbara
• Primary Examiner(s): SAUNDERS, DAVID A
• Application Number:
  US09/643,839
• Time in Patent Office: 834 Days
• Field of Search: 435/2, 435/7.21, 435/29, 435/325, 435/30, 530/388.2, 530/389.2
• US Class Current: 435/2
• CPC Class Codes: C12N 5/0612 sorting of gametes, e.g. according to sex or motility
• CPC Class Codes: G01N 33/54326 Magnetic particles
• CPC Class Codes: G01N 33/56966 Animal cells
• CPC Class Codes: Y10S 530/852 Sperm

Abstract

A method for increasing the percentage of mammalian offspring of either sex which comprises contacting a semen sample with an antibody specific for the spermatozoa determinative of one sex and separating said spermatozoa from spermatozoa determinative of the other sex, said antibody being bound to a non-porous magnetic bead support having a diameter of 0.1 to 2 microns.

Claims

1. A method for separating spermatozoa determinative of one sex from spermatozoa determinative of the other sex comprising contacting a semen sample with antibody specific for a spermatozoa determinative of one sex, and magnetic beads having a diameter of 0.1 to 2 microns, and separating spermatozoa bound to said antibody and linked to said beads from said sample.

2. The method according to claim 1, wherein said antibody is linked to said beads through a linking compound.

3. The method according to claim 2, wherein said linking compound is an antibody.

4. The method according to claim 3, wherein said linking antibody is IgG.

5. The method according to claim 2, wherein said linking compound is protein A.

6. The method according to claim 5, wherein said semen sample is contacted with said beads, the sample is exposed to a magnetic field, and the remaining supernatant is removed to provide a supernatant rich in X-bearing sperm.

7. The method according to claim 6, wherein said supernatant rich in X-bearing sperm is subjected to said method at least one additional time.

8. The method according to claim 1, wherein said antibody specific for said spermatozoa determinative of one sex is IgG.

9. The method according to any one of claims 1-5 or 8, wherein said antibody specific for said spermatozoa determinative of one sex is specific for Y-bearing sperm.

10. The method according to claim 9, wherein said antibody specific for Y-bearing sperm is a polyclonal antibody.

11. The method according to claim 9, wherein said antibody specific for Y-bearing sperm is specific for an H-Y antigen.

12. The method according to claim 9, wherein said beads have a diameter of 0.1 to 0.5 microns.

13. The method according to claim 12, wherein said antibody specific for Y-bearing sperm is a monoclonal antibody.

14. The method according to any one of claims 1-5 or 8, wherein said antibody specific for said spermatozoa determinative of one sex is specific for X-bearing sperm.

15. The method according to claim 14, wherein said beads have a diameter of 0.1 to 0.5 microns.

16. The method according to claim 15, wherein said antibody specific for X-bearing sperm is a monoclonal antibody.

17. The method according to claim 14, wherein said antibody specific for X-bearing sperm is a polycolonal antibody.

18. The method according to claim 1, wherein said semen sample is contacted with said beads, the sample is exposed to a magnetic field, and the remaining supernatant is removed to provide a supernatant rich in Y-bearing sperm.