Method for culturing a basidiomycetous fungus in a liquid culture medium
First Claim
1. A method for culturing a basidiomycetous fungus in an aqueous liquid culture medium which comprises the steps of:
- (a) inoculating a liquid culture medium containing inorganic nutrient salts for nitrogen, phosphate and potassium with a body of the fungus;
(b) admixing the liquid culture medium with crude cane sugar in an amount in the range from 50 g to 70 g calculated as sucrose per liter of the liquid culture medium;
(c) admixing the liquid culture medium with a water-insoluble growth-supporting material selected from the group consisting of crushed sugarcane, sugarcane bagasse, pine tree-tissue and wheat bran in an amount in the range from 0.2 g to 15 g as dry per liter of the liquid culture medium;
(d) keeping the liquid culture medium under agitation at a temperature in the range from 20 to 30 °
C.; and
(e) blowing, into the liquid culture medium, oxygen-enriched air containing at least 30% by volume of oxygen under a pressure in the range from 0.12 to 0.5 MPa (absolute) at a rate of at least 0.01 liter/minute per liter of the liquid culture medium.
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Abstract
Disclosed is an efficient method for culturing an edible basidiomycetous fungus such as Mushroom Agaricus Blazei Murill in a liquid culture medium to give fungus aggregates of several centimeter size. Characteristically, the liquid culture medium is formulated with sucrose as a carbon source in the form of crude cane sugar in combination with a water-insoluble growth-supporting material in the form of a fine powder to serve as the core of the fungus aggregates as selected from crushed sugarcane, sugarcane bagasse, pine trees and wheat bran. Further characteristically, the culturing procedure is carried out under an oxygen-enriched condition by blowing oxygen-enriched air of at least 30% by volume oxygen into the culture medium under pressurization at 0.12 to 0.5 MPa (absolute) in a specified blowing rate.
48 Citations
6 Claims
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1. A method for culturing a basidiomycetous fungus in an aqueous liquid culture medium which comprises the steps of:
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(a) inoculating a liquid culture medium containing inorganic nutrient salts for nitrogen, phosphate and potassium with a body of the fungus;
(b) admixing the liquid culture medium with crude cane sugar in an amount in the range from 50 g to 70 g calculated as sucrose per liter of the liquid culture medium;
(c) admixing the liquid culture medium with a water-insoluble growth-supporting material selected from the group consisting of crushed sugarcane, sugarcane bagasse, pine tree-tissue and wheat bran in an amount in the range from 0.2 g to 15 g as dry per liter of the liquid culture medium;
(d) keeping the liquid culture medium under agitation at a temperature in the range from 20 to 30 °
C.; and
(e) blowing, into the liquid culture medium, oxygen-enriched air containing at least 30% by volume of oxygen under a pressure in the range from 0.12 to 0.5 MPa (absolute) at a rate of at least 0.01 liter/minute per liter of the liquid culture medium. - View Dependent Claims (2, 3, 4, 5, 6)
(f) controlling the concentration of carbon dioxide in the gaseous phase over the liquid culture medium.
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Specification
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- Resources
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Current AssigneeTsukuba Biosystem, Ltd.
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Original AssigneeTsukuba Biosystem, Ltd.
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InventorsIntabon, Keo, Maekawa, Takaaki
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Primary Examiner(s)Jordan, Charles T.
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Assistant Examiner(s)HAYES, BRET C
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Application NumberUS09/958,664Time in Patent Office424 DaysField of Search47/11, 47/14US Class Current47/1.1CPC Class CodesA01G 18/20 Culture media, e.g. compostA01G 18/50 Inoculation of spawnC12M 27/02 Stirrer or mobile mixing el...C12M 29/06 Nozzles; Sprayers; Spargers...C12M 41/40 of pressureC12N 1/14 Fungi culture of mushrooms ...
