Test strip and biosensor incorporating with nanometer metal particles

• US 6,491,803 B1
• Filed: 05/18/2001
• Issued: 12/10/2002
• Est. Priority Date: 05/18/2001
• Status: Active Grant

• Current Assignee: Apex Biotechnology Corporation
• Original Assignee: Apex Biotechnology Corporation
• Inventors: Chuang, Jen-Hung, Lin, Hong-Ming, Chen, Wen-Chang, Shen, Thomas Y. S.
• Primary Examiner(s): Tung, T.
• Assistant Examiner(s): Noguerola, Alex
• Application Number:
  US09/859,371
• Time in Patent Office: 571 Days
• Field of Search: 204/403, 204/403.01, 204/403.06, 204/403.1, 204/403.11, 427/2.13
• US Class Current: 204/403.11
• CPC Class Codes: G01N 2333/904 acting on CHOH groups as donors, e.g. glucose oxidase, lactate dehydrogenase (1.1)
• CPC Class Codes: G01N 27/3272 Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels optical biosensors G01N33/52
• CPC Class Codes: G01N 33/5438 Electrodes

Abstract

The present invention relates to a test strip and a biosensor having an increased conductivity and a slurry comprising a fiber, meal particles having a size in nanometer and a bioactive substance. The invention is characterized by incorporating metal particles having size in nanometer into the reaction layer of test strip and biosensor to increase the conductivity between the reaction layer and the electrodes so that the redox reaction can be readily completed and the measurement time can thus be shortened.

Claims

1. A test strip, which comprises:

an electrically insulating substrate;

a conducting film coated on one side of said insulating substrate to form an isolated and disconnected anode, and an isolated and disconnected cathode wherein said anode is disposed on the substrate, the anode being formed with, on both ends of the anode, a working electrode and an anode connector respectively; and

said cathode is disposed on the substrate, the cathode being formed with, on both ends of the cathode, a reference electrode and a cathode connector respectively;

a reaction layer wherein said layer is formed by coating the working electrode and the reference electrode with a slurry comprising a fiber, metal particles having a size in nanometer, and a bioactive substance, potassium ferricyanide as electrical mediator, a polymer and a surfactant; and

an electrically insulating layer disposed on the substrate and having an opening for receiving the sample on the reaction layer.

2. The test strip of claim 1, wherein said substrate is selected from the group consisting of PVC, glass fiber, polyester suphone, bakelite plate, PET, PC, glass and ceramic board.

3. The test strip of claim 2, wherein the particle size of said fiber ranges from 20-100 μ

m in diameter.

4. The test strip of claim 1, wherein said fiber is selected from the group consisting of cellulose, microcellulose, methylcellulose, carboxylmethyl-cellulose, starch, vinylalcohol, vinylpyrrolidone, PVA, PVP, PEG and gelatin.

5. The test strip of claim 1, wherein said metal particles are selected from the group consisting of Fe, Co, Cu, Ag, Au, Ni, Pd, Pt and the mixture, alloys, or nanocomposites containing these nanometer metal particles thereof.

6. The test strip of claim 5, wherein the size of said metal particles ranges from 1-100 nm in diameter.

7. The test strip of claim 1, wherein said bioactive material is selected from the group consisting of enzyme, antigen, antibody, microorganism, animal cell, plant cell, animal or plant tissue with the component having biological recognition ability.

8. The test strip of claim 7, wherein said bioactive material is an enzyme.

9. The test strip of claim 8, wherein said enzyme is an immobilized enzyme.

10. The test strip of claim 8, wherein said enzyme is glucose oxidase.

11. The test strip of claim 1, wherein said slurry further comprises a salt and water.

12. The test strip of claim 11, wherein the electrical mediator is a potassium ferricyanide.

13. The test strip of claim 1, wherein the polymer is selected from the group consisting of polyvinyl alcohol, polyvinyl pyrrolidone, polyethylene glycol, gelatin and the mixture thereof.

14. The test strip of claim 1, wherein the surfactant is selected from the group consisting of Triton X-100, Triton C-405, Triton X-114, sodium lauryl sulfate, polyoxyethylenesorbitan monolaurate (Tween 20), Tween 40, Tween 60, Tween 80, or other water-soluble surfactant or detergent.

15. A method of producing said test strip of claim 14, which comprises the following steps:

(a) coating a conducting film on one side of an electric insulating substrate and forming isolated and disconnected an anode and a cathode;

(b) coating an electric insulating film on a part of the conducting film, wherein one end of an uncovered anode of the conducting film is at least a reference electrode and the other end an anode connector, and one end of an uncovered cathode of the conducting fit is at least a working electrode and the other end a cathode connector; and

(c) coating a region containing at least the working electrode, with a the slurry comprising a fiber, metal particles having a size in nanometer and a bioactive substance, and the reference electrode to form a reaction film so as to connect the working electrode and the reference electrode individually.

16. A biosensor, which comprises the test strip of claim 1 and a detection device.

17. The biosensor of claim 16, wherein the detection device is an amperometric sensor.