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Vertebrate smoothened proteins

  • US 6,492,139 B1
  • Filed: 04/27/2000
  • Issued: 12/10/2002
  • Est. Priority Date: 09/30/1996
  • Status: Expired due to Term
First Claim
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1. Isolated nucleic acid which hybridizes to either a) SEQ ID NO:

  • 1 or b) the complement thereof, wherein said nucleic acid encodes vertebrate Smoothened which directly binds to Patched, and wherein said hybridization occurs under the following conditions;

    42°

    C. in 50% formamide, 5×

    SSC, 10×

    Denhardt'"'"'s, 0.05M sodium phosphate, (pH 6.5), 0.1% sodium pyrophosphate, 50 μ

    g/ml sonicated salmon sperm, followed by rinsing in 2×

    SSC and washing with 0.5×

    SSC and 0.1% SDS at 42°

    C.

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