Vertebrate smoothened proteins
First Claim
Patent Images
1. Isolated nucleic acid which hybridizes to either a) SEQ ID NO:
- 1 or b) the complement thereof, wherein said nucleic acid encodes vertebrate Smoothened which directly binds to Patched, and wherein said hybridization occurs under the following conditions;
42°
C. in 50% formamide, 5×
SSC, 10×
Denhardt'"'"'s, 0.05M sodium phosphate, (pH 6.5), 0.1% sodium pyrophosphate, 50 μ
g/ml sonicated salmon sperm, followed by rinsing in 2×
SSC and washing with 0.5×
SSC and 0.1% SDS at 42°
C.
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Abstract
Novel vertebrate homologues of Smoothened, including human and rat Smoothened, are provided. Compositions including vertebrate Smoothened chimeras, nucleic acid encoding vertebrate Smoothened, and antibodies to vertebrate Smoothened, are also provided.
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Citations
28 Claims
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1. Isolated nucleic acid which hybridizes to either a) SEQ ID NO:
- 1 or b) the complement thereof, wherein said nucleic acid encodes vertebrate Smoothened which directly binds to Patched, and wherein said hybridization occurs under the following conditions;
42°
C. in 50% formamide, 5×
SSC, 10×
Denhardt'"'"'s, 0.05M sodium phosphate, (pH 6.5), 0.1% sodium pyrophosphate, 50 μ
g/ml sonicated salmon sperm, followed by rinsing in 2×
SSC and washing with 0.5×
SSC and 0.1% SDS at 42°
C. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8)
- 1 or b) the complement thereof, wherein said nucleic acid encodes vertebrate Smoothened which directly binds to Patched, and wherein said hybridization occurs under the following conditions;
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9. Isolated nucleic acid which hybridizes to either a) SEQ ID NO:
- 3 or b) the complement thereof, wherein said nucleic acid encodes vertebrate Smoothened which directly binds to Patched, and wherein said hybridization occurs under the following conditions;
42°
C. in 50% formamide, 5×
SSC, 10×
Denhardt'"'"'s, 0.05M sodium phosphate, (pH 6.5), 0.1% sodium pyrophosphate, 50 μ
g/ml sonicated salmon sperm, followed by rinsing in 2×
SSC and washing with 0.5×
SSC and 0.1% SDS at 42°
C. - View Dependent Claims (10, 11, 12, 13, 14, 15, 16)
- 3 or b) the complement thereof, wherein said nucleic acid encodes vertebrate Smoothened which directly binds to Patched, and wherein said hybridization occurs under the following conditions;
- 17. Isolated nucleic acid comprising the coding sequence for human vertebrate Smoothened polypeptide deposited under ATTC Dep. No. 98162.
- 19. Isolated nucleic acid comprising the coding sequence for human vertebrate Smoothened polypeptide deposited under ATTC Dep. No. 98163.
- 21. Isolated nucleic acid comprising the coding sequence for rat nucleic acid deposited under ATCC Dep. No. 98165.
- 23. A nucleic acid comprising a nucleotide sequence encoding a polypeptide comprising residues 30 to 787 of FIG. 4 (SEQ ID NO:
- 26. A nucleic acid comprising a nucleotide sequence that encodes residues 33 to 793 of FIG. 1 (SEQ ID NO:
Specification