Method for improving the performance of a photobioreactor

• US 6,492,149 B1
• Filed: 04/17/2001
• Issued: 12/10/2002
• Est. Priority Date: 10/19/1998
• Status: Expired due to Fees

• Current Assignee: Institut francais de recherche pour l'exploitation de la mer
• Original Assignee: Institut francais de recherche pour l'exploitation de la mer
• Inventors: Muller-Feuga, Arnaud
• Primary Examiner(s): TATE, CHRISTOPHER ROBIN
• Application Number:
  US09/807,666
• Time in Patent Office: 602 Days
• Field of Search: 435/292.1, 435/420, 435/260, 435/173.8, 435/257.1
• US Class Current: 435/173.8
• CPC Class Codes: C12M 21/02 Photobioreactors culturing algae A01G33/00, A01H4/001, C12N1/12
• CPC Class Codes: C12M 23/06 Tubular C12M23/08, C12M23/16 take precedence
• CPC Class Codes: C12M 31/08 by conducting or reflecting elements located inside the reactor or in its structure
• CPC Class Codes: C12M 41/36 of biomass, e.g. colony counters or by turbidity measurements electrooptical investigation of individual particles G01N15/14, flow cytometers G01N15/1404

Abstract

The invention relates to a method of improving the yield of a photobioreactor of the continuously operating recirculation type as a function of the species of micro-organism being cultured, of the thickness d of the chambers (10) of the reactor, and of the final concentration desired for the culture. In the method, transparent or respecting particles (15) are introduced into the reaction medium (11), which particles are of a density that is substantially equal to that of the reaction medium, thereby adjusting absorbance of the microorganism culture to optimum levels. The invention is mainly applicable to culturing phototropic micro-organisms.

Claims

1. A method for improving a rate of growth of phototropic microorganisms in a culture in suspension in a liquid reaction medium circulating in a chamber of a photobioreactor, said chamber having a transparent wall through which pass radiation for activating the photosynthesis reaction of the phototropic microorganisms, the method comprising:

adding transparent or reflecting particles to the reaction medium in order to adjust radiation absorbance of the culture, the density of said particles being substantially equal to the density of the medium; and

the volume percentage of said particles in the reaction medium being a function of the species of microorganism in the culture, the thickness of the chamber that corresponds to an optical path of the radiation, and the desired final concentration of the culture in the reaction medium.

2. A method according to claim 1, in which the reaction medium is circulated in a closed circuit, an inoculum is introduced into said closed circuit, and a portion of the reaction medium is extracted once the concentration of the culture has reached the desired threshold, the method including measuring the turbidity of the reaction medium and adjusting the intensity of the radiation as a function of the measured turbidity.

3. A method according to claim 1, wherein the particles are compact in shape so as to avoid effects of lift and of superposition.

4. A method according to claim 1, wherein the particles have a diameter in the range of 0.5 mm to 10 mm.

5. A method according to claim 1, wherein the particles used are made by coating hollow glass microbeads in a transparent plastics material at proportions such that the density of the resulting composite is equal to that of the reaction medium.