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Methods for producing members of specific binding pairs

  • US 6,492,160 B1
  • Filed: 06/25/1998
  • Issued: 12/10/2002
  • Est. Priority Date: 05/15/1991
  • Status: Expired due to Fees
First Claim
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1. Recombinant vectors produced by a method which comprises:

  • causing or allowing recombination between (a) first vectors comprising nucleic acid encoding a population of first polypeptide chain of a specific binding pair member; and

    (b) second vectors comprising nucleic acid encoding a population of a second polypeptide chain of a specific binding pair member, at least one of said populations being genetically diverse, the recombination resulting in recombinant vectors each of which comprises nucleic acid encoding a said first polypeptide chain and a said second polypeptide chain, the recombination being promoted by inclusion in said first and second vectors of sequences at which site-specific recombination occurs, each of the first vectors and each of the second vectors including a first site-specific recombination sequence and a second site-specific recombination sequence different from the first, site-specific recombination taking place preferentially between first site-specific recombination sequences on different vectors and between second site-specific recombination sequences on different vectors compared with a first site-specific recombination sequence and a second site-specific recombination sequence on the same vector.

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