Nucleic acids encoding extracellular matrix proteins

• US 6,492,508 B1
• Filed: 10/09/1998
• Issued: 12/10/2002
• Est. Priority Date: 06/03/1996
• Status: Expired due to Fees

• Current Assignee: United States Surgical Corporation (Medtronic Plc)
• Original Assignee: United States Surgical Corporation (Medtronic Plc)
• Inventors: Zhang, Guanghui, Connelly, Kevin, Buechter, Douglas D., Gruskin, Elliott A.
• Primary Examiner(s): Myers, Carla J.
• Application Number:
  US09/169,768
• Time in Patent Office: 1,523 Days
• Field of Search: 536/23.1, 536/23.5, 530/350, 530/356, 435/69.1, 435/71.1, 435/71.2, 435/440, 435/471
• US Class Current: 536/23.5
• CPC Class Codes: C07K 14/4725 Proteoglycans, e.g. aggreccan
• CPC Class Codes: C07K 14/495 Transforming growth factor [TGF]
• CPC Class Codes: C07K 14/51 Bone morphogenetic factor; Osteogenins; Osteogenic factor; Bone-inducing factor
• CPC Class Codes: C07K 14/75 Fibrinogen
• CPC Class Codes: C07K 14/78 Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin or cold insoluble globulin [CIG]
• CPC Class Codes: C07K 2319/00 Fusion polypeptide
• CPC Class Codes: C12P 21/02 having a known sequence of two or more amino acids, e.g. glutathione

Abstract

Incorporation of certain amino acid analogs into polypeptides produced by cells which do not ordinarily provide polypeptides containing such amino acid analogs is accomplished by subjecting the cells to growth media containing such amino acid analogs. The degree of incorporation can be regulated by adjusting the concentration of amino acid analogs in the media and/or by adjusting osmolality of the media. Such incorporation allows the chemical and physical characteristics of polypeptides to be altered and studied. In addition, nucleic acid and corresponding proteins including a domain from a physiologically active peptide and a domain from an extracellular matrix protein which is capable of providing a self-aggregate are provided. Human extracellular matrix proteins capable of providing a self-aggregate collagen are provided which are produced by prokaryotic cells. Preferred codon usage is employed to produce extracellular matrix proteins in prokaryotics.

Claims

1. Nucleic acid comprising the sequence shown in SEQ. ID. NO. 19.

2. Nucleic acid comprising the sequence shown in SEQ. ID. NO. 43.