Method for developing transgenic mice
First Claim
Patent Images
1. A method for producing a transgenic mouse with a desired genotype, said method comprising:
- (a) obtaining a mouse tetraploid blastocyst;
(b) introducing a plurality of mouse totipotent cells into said tetraploid blastocyst, wherein said totipotent cells have been genetically manipulated to comprise a transgene conferring a desired genotype to said cells, thereby producing a transgenic embryo; and
(c) transferring said embryo into a foster mother under conditions favoring the development of said embryo into a transgenic mouse comprising said desired genotype.
1 Assignment
0 Petitions
Accused Products
Abstract
Process for producing mammals with defined genetic properties, particularly transgenic mammals. Animals which are derived completely from ES cells are obtained by injecting totipotent cells (embryonic stem cells or embryonic germ cells) genetically manipulated and cultured in vitro into tetraploid blastocysts and implanting the resulting embryo in a foster mother, in a single operation.
-
Citations
14 Claims
-
1. A method for producing a transgenic mouse with a desired genotype, said method comprising:
-
(a) obtaining a mouse tetraploid blastocyst;
(b) introducing a plurality of mouse totipotent cells into said tetraploid blastocyst, wherein said totipotent cells have been genetically manipulated to comprise a transgene conferring a desired genotype to said cells, thereby producing a transgenic embryo; and
(c) transferring said embryo into a foster mother under conditions favoring the development of said embryo into a transgenic mouse comprising said desired genotype. - View Dependent Claims (2, 3, 4, 5, 6, 7)
-
-
8. A method for producing a transgenic mouse, comprising:
-
(a) obtaining a mouse tetraploid blastocyst by electrofusion of two-cell diploid embryos obtained from mouse;
(b) genetically manipulating mouse embryonic stem cells, wherein said genetic manipulation introduces a transgene into said embryonic stem cells; and
(c) introducing one or more of said embryonic stem cells into said tetraploid blastocyst, thereby generating a transgenic embryo; and
(d) implanting said transgenic embryo into a foster mother under conditions favoring development of said embryo into a transgenic mouse. - View Dependent Claims (9, 10, 11, 12, 13, 14)
-
Specification