Electrochemical methods for generation of a biological proton motive force and pyridine nucleotide cofactor regeneration
First Claim
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1. A method of promoting reductive processes in a biological system comprising the steps of:
- (a) providing an electrochemical bioreactor system having a cathode compartment equipped with a cathode and an anode compartment equipped with an anode, the cathode and anode compartment being separated by a cation selective membrane, wherein the cathode and anode are connected by a conductive material to a power supply;
(b) placing a suitable amount of neutral red and a biological catalyst in the cathode compartment; and
(c) applying from the power supply a current between the anode and the cathode wherein at least a portion of the neutral red is electrically reduced, wherein the biological catalyst comprises Trichosporon capitatum.
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Abstract
Disclosed are methods using neutral red to mediate the interconversion of chemical and electrical energy. Electrically reduced neutral red has been found to promote cell growth and formation of reduced products by reversibly increasing the ratio of the reduced:oxidized forms of NAD(H) or NADP(H). Electrically reduced neutral red is able to serve as the sole source of reducing power for microbial, cell growth. Neutral red is also able to promote conversion of chemical energy to electrical energy by facilitating the transfer of electrons from microbial reducing power to a fuel cell cathode.
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Citations
18 Claims
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1. A method of promoting reductive processes in a biological system comprising the steps of:
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(a) providing an electrochemical bioreactor system having a cathode compartment equipped with a cathode and an anode compartment equipped with an anode, the cathode and anode compartment being separated by a cation selective membrane, wherein the cathode and anode are connected by a conductive material to a power supply;
(b) placing a suitable amount of neutral red and a biological catalyst in the cathode compartment; and
(c) applying from the power supply a current between the anode and the cathode wherein at least a portion of the neutral red is electrically reduced, wherein the biological catalyst comprises Trichosporon capitatum. - View Dependent Claims (2, 3, 4, 5, 6)
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7. A method of promoting reductive processes in a biological system comprising the steps of:
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(a) providing an electrochemical bioreactor system having a cathode compartment equipped with a cathode and an anode compartment equipped with an anode, the cathode and anode compartment being separated by a cation selective membrane, wherein the cathode and anode are connected by a conductive material to a power supply;
(b) placing a suitable amount of neutral red, NAD(H) or NADP(H), and a biological catalyst in the cathode compartment; and
(c) applying from the power supply a current between the anode and the cathode wherein at least a portion of the neutral red is electrically reduced, wherein the biological catalyst comprises a β
-tetralone reductase.- View Dependent Claims (8, 9, 10, 11, 12)
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13. A method of promoting reductive processes in a biological system comprising the steps of:
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(a) providing an electrochemical bioreactor system having a cathode compartment equipped with a cathode and an anode compartment equipped with an anode, the cathode and anode compartment being separated by a cation selective membrane, wherein the cathode and anode are connected by a conductive material to a power supply;
(b) placing a suitable amount of neutral red, 6-bromo-β
-tetralone, and a biological catalyst in the cathode compartment; and
(c) applying from the power supply a current between the anode and the cathode wherein at least a portion of the neutral red is electrically reduced. - View Dependent Claims (14, 15, 16, 17, 18)
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Specification