PRRSV antigenic sites identifying peptide sequences of PRRS virus for use in vaccines or diagnostic assays

• US 6,495,138 B1
• Filed: 11/05/1999
• Issued: 12/17/2002
• Est. Priority Date: 05/06/1997
• Status: Expired due to Term

• Current Assignee: Boehringer Ingelheim Vetmedica GmbH (C.H. Boehringer Sohn AG & Co. KG)
• Original Assignee: Stichting Dienst Landbouwkundig Onderzoek
• Inventors: van Nieuwstadt, Antonie Paul, Langeveld, Jan, Meulenberg, Janneke
• Primary Examiner(s): SALIMI, ALI REZA
• Application Number:
  US09/434,476
• Time in Patent Office: 1,138 Days
• Field of Search: 424/186.1, 424/204.1, 424/211.1, 536/23.72, 530/327, 530/326, 530/328
• US Class Current: 424/186.1
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: A61K 39/00 Medicinal preparations containing antigens or antibodies materials for immunoassay G01N33/53
• CPC Class Codes: A61K 39/12 Viral antigens
• CPC Class Codes: A61P 31/14 for RNA viruses
• CPC Class Codes: C07K 14/005 from viruses
• CPC Class Codes: C07K 16/10 from RNA viruses
• CPC Class Codes: C07K 2317/34 Identification of a linear epitope shorter than 20 amino acid residues or of a conformational epitope defined by amino acid residues
• CPC Class Codes: C12N 2770/10022 New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
• CPC Class Codes: C12N 2770/10034 Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein

Abstract

The invention provides antigenic sites of PRRSV isolates. The antigenic sites are neutralizing, conserved, non-conserved and conformational, can elicit antibodies and are found on protein GP4 and N encoded ORF4 and ORF7 of PRRSV. The peptide sequences identified by the sites can be incorporated in vaccines directed against PRRS and in diagnostic tests for PRRS. Also, discriminating tests can be developed that can be used next to marker vaccines in programs designed to eradicate PRRS from pig herds.

Claims

1. A synthetic peptide that elicits antibodies that react with at least two different PRRSV isolates, said synthetic peptide comprising an amino acid sequence of at least 10 to about 15 amino acid residues completely overlapping a conserved site of protein N of PRRSV wherein said conserved site comprises the amino acid residues located at amino acid position 22 to 32 of protein N of PRRSV isolate I-1102.

2. The synthetic peptide of claim 1, wherein one or more of said amino acid residues of said amino acid sequence have been replaced while retaining said synthetic peptide'"'"'s ability to elicit said antibodies.

3. A synthetic peptide that elicits antibodies which antibodies react with at least two different PRRSV isolates, said synthetic peptide comprising an amino acid sequence of at least about 5 to about 15 amino acid residues partly or completely overlapping a conserved site of protein N of PRRSV wherein said amino acid sequence is selected from the group consisting of SEQ ID NO. 1 and SEQ ID NO. 2.