In-line complete spectral fluorescent imaging of nucleic acid molecules

• US 6,495,363 B2
• Filed: 05/18/2001
• Issued: 12/17/2002
• Est. Priority Date: 08/18/1998
• Status: Expired due to Term

• Current Assignee: Beckman Coulter Incorporated (Danaher Corporation)
• Original Assignee: Orchid Biosciences Incorporated (Laboratory Corporation Of America Holdings)
• Inventors: Bogdanov, Valery
• Primary Examiner(s): Shaw, Jeffrey
• Application Number:
  US09/861,137
• Publication Number:
  US 20020009744A1
• Time in Patent Office: 578 Days
• Field of Search: 435/6, 435/7.1, 435/91.2, 435/287.2, 536/22.1, 536/23.1, 536/24.3, 536/24.31, 536/24.32, 536/24.33, 250/334, 204/461
• US Class Current: 506/39
• CPC Class Codes: G01N 2021/6423 Spectral mapping, video display
• CPC Class Codes: G01N 21/6428 Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53
• CPC Class Codes: G01N 21/6452 Individual samples arranged in a regular 2D-array, e.g. multiwell plates
• CPC Class Codes: G01N 21/6456 Spatial resolved fluorescence measurements; Imaging

Abstract

The present invention provides a hyperspectral imaging apparatus and methods for employing such an apparatus for multi-dye/base detection of a nucleic acid molecule coupled to a solid surface.

Claims

1. A hyperspectral fluorescent imaging apparatus for microarray detection which comprises:

(a) a light source, wherein the light source is capable of emitting a transmission beam for hyperspectral fluorescent imaging;

(b) an expansion lens that expands the transmission beam for microarray detection;

(c) a focusing lens that focuses the expanded transmission beam into a thin focus line for microarray detection;

(d) a translation stage;

(e) a collection lens that collects fluorescent light emitted at the thin focus line;

an imaging spectrometer for hyperspectral fluorescent imaging; and

(g) a detector.

3. A hyperspectral fluorescent imaging apparatus according to claim 1, wherein the detector is a charge coupled device.

4. A hyperspectral fluorescent imaging apparatus according to claim 1, wherein the light source is a laser.

5. A hyperspectral fluorescent imaging apparatus according to claim 4, wherein the laser is a CW Ar laser.

6. A hyperspectral fluorescent imaging apparatus according to claim 1, wherein the apparatus comprises a first expansion lens and a second expansion lens.

2. A hyperspectral fluorescent imaging apparatus for microarray detection which comprises:

(a) a light source, wherein the light source is capable of emitting a transmission beam for hyperspectral fluorescent imaging;

(b) a first expansion lens and a second expansion lens that expand the transmission beam for microarray detection;

(c) a focusing lens that focuses the expanded transmission beam into a thin focus line for microarray detection;

(d) a collection lens that collects fluorescent light emitted at the thin focus line;

(e) an imaging spectrometer for hyperspectral fluorescent imaging; and

(f) a detector.

7. A hyperspectral fluorescent imaging apparatus according to claim 2, wherein the apparatus further comprises a translation stage.

8. A hyperspectral fluorescent imaging apparatus according to claim 2, wherein the detector is charge coupled device.

9. A hyperspectral fluorescent imaging apparatus according to claim 2, wherein the light source is a laser.

10. A hyperspectral fluorescent imaging apparatus according to claim 9, wherein the laser is a CW Ar laser.