In-line complete spectral fluorescent imaging of nucleic acid molecules
First Claim
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1. A hyperspectral fluorescent imaging apparatus for microarray detection which comprises:
- (a) a light source, wherein the light source is capable of emitting a transmission beam for hyperspectral fluorescent imaging;
(b) an expansion lens that expands the transmission beam for microarray detection;
(c) a focusing lens that focuses the expanded transmission beam into a thin focus line for microarray detection;
(d) a translation stage;
(e) a collection lens that collects fluorescent light emitted at the thin focus line;
an imaging spectrometer for hyperspectral fluorescent imaging; and
(g) a detector.
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Abstract
The present invention provides a hyperspectral imaging apparatus and methods for employing such an apparatus for multi-dye/base detection of a nucleic acid molecule coupled to a solid surface.
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Citations
10 Claims
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1. A hyperspectral fluorescent imaging apparatus for microarray detection which comprises:
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(a) a light source, wherein the light source is capable of emitting a transmission beam for hyperspectral fluorescent imaging;
(b) an expansion lens that expands the transmission beam for microarray detection;
(c) a focusing lens that focuses the expanded transmission beam into a thin focus line for microarray detection;
(d) a translation stage;
(e) a collection lens that collects fluorescent light emitted at the thin focus line;
an imaging spectrometer for hyperspectral fluorescent imaging; and
(g) a detector. - View Dependent Claims (3, 4, 5, 6)
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2. A hyperspectral fluorescent imaging apparatus for microarray detection which comprises:
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(a) a light source, wherein the light source is capable of emitting a transmission beam for hyperspectral fluorescent imaging;
(b) a first expansion lens and a second expansion lens that expand the transmission beam for microarray detection;
(c) a focusing lens that focuses the expanded transmission beam into a thin focus line for microarray detection;
(d) a collection lens that collects fluorescent light emitted at the thin focus line;
(e) an imaging spectrometer for hyperspectral fluorescent imaging; and
(f) a detector. - View Dependent Claims (7, 8, 9, 10)
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Specification