Oligonucleotide and nucleotide amine analogs, methods of synthesis and use
First Claim
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1. A method for detecting the presence or absence of an RNA in a biological sample suspected of containing said RNA comprising:
- contacting a sample with a compound having the structure;
wherein R1 and R2 are independently H, a nucleotide, oligonucleotide or polyamine species and at least one of R1 and R2 is a purine containing oligonucleotide, R3 is a linear or cyclic non-aromatic polyamine species, and X is H, O—
R11, S—
R11, F, Cl, Br, CN, CF3, OCF3, OCN, SOCH3, SO2CH3, ONO2, N3, HN2, heterocylcoalkyl, heterocycloalkaryl, aminoalkylamino, polyalkylamino, substituted silyl, a reporter molecule, an RNA cleaving group, wherein R11 is H, C1 to C10 straight or branched chain lower alkyl or substituted lower alkyl, C2 to C10 straight or branched chain lower alkenyl or substituted lower alkyenyl, C3 to C10 straight or branched chain lower alkynyl or substituted lower alkynyl, a 14C containing lower alkyl, lower alkenyl or lower alkynyl, C7 to C14 substituted or unsubstituted alkyaryl or aralkyl, a 14C containing C7 to C14 alkaryl or aralkyl, alicyclic, heterocyclic, a reporter molecule, a RNA cleaving group;
said compound being specifically hybridizable with said RNA; and
detecting the presence or absence of hybridization of the compound to the sample wherein hybridization is indicative of the presence of RNA in the sample.
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Abstract
Novel amine compounds are provided by the present invention. Methods of preparing and using said novel amine compounds are also provided.
16 Citations
4 Claims
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1. A method for detecting the presence or absence of an RNA in a biological sample suspected of containing said RNA comprising:
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contacting a sample with a compound having the structure;
wherein R1 and R2 are independently H, a nucleotide, oligonucleotide or polyamine species and at least one of R1 and R2 is a purine containing oligonucleotide, R3 is a linear or cyclic non-aromatic polyamine species, and X is H, O—
R11, S—
R11, F, Cl, Br, CN, CF3, OCF3, OCN, SOCH3, SO2CH3, ONO2, N3, HN2, heterocylcoalkyl, heterocycloalkaryl, aminoalkylamino, polyalkylamino, substituted silyl, a reporter molecule, an RNA cleaving group, wherein R11 is H, C1 to C10 straight or branched chain lower alkyl or substituted lower alkyl, C2 to C10 straight or branched chain lower alkenyl or substituted lower alkyenyl, C3 to C10 straight or branched chain lower alkynyl or substituted lower alkynyl, a 14C containing lower alkyl, lower alkenyl or lower alkynyl, C7 to C14 substituted or unsubstituted alkyaryl or aralkyl, a 14C containing C7 to C14 alkaryl or aralkyl, alicyclic, heterocyclic, a reporter molecule, a RNA cleaving group;
said compound being specifically hybridizable with said RNA; and
detecting the presence or absence of hybridization of the compound to the sample wherein hybridization is indicative of the presence of RNA in the sample. - View Dependent Claims (2, 3, 4)
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Specification
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Current AssigneeISIS Pharmaceuticals Incorporated
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Original AssigneeISIS Pharmaceuticals Incorporated
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InventorsManoharan, Muthiah, Cook, P. Dan
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Primary Examiner(s)FREDMAN, JEFFREY NORMAN
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Application NumberUS09/689,964Time in Patent Office796 DaysField of Search536/23.1, 536/25.3, 536/24.31, 536/24.3, 536/22.1, 435/6US Class Current536/23.1CPC Class CodesC07H 21/00 Compounds containing two or...C12Q 1/6813 Hybridisation assays
