Method and apparatus for DNA sequencing

• US 6,497,804 B1
• Filed: 12/03/1999
• Issued: 12/24/2002
• Est. Priority Date: 12/03/1998
• Status: Expired due to Term

• Current Assignee: The Research Foundation for The State University of New York (State University of New York)
• Original Assignee: The Research Foundation for The State University of New York (State University of New York)
• Inventors: Gorfinkel, Vera, Gouzman, Mikhail, Serge, Luryi
• Primary Examiner(s): Warden, Jill
• Assistant Examiner(s): BROWN, JENNINE M
• Application Number:
  US09/454,093
• Time in Patent Office: 1,117 Days
• Field of Search: 204/603, 435/6
• US Class Current: 204/603
• CPC Class Codes: G01N 27/44721 by optical means

Abstract

The development of the network structure and the basic modules of an automated 4-color DNA sequencing apparatus comprising more than one thousand capillary electrophoresis lanes is disclosed. The basic modules represent small 32-lane units based on multicolor excitation of fluorescent labels and single-photon detection. The individual units operate asynchronously, controlled by a network computer. Excitation of fluorescence is done with low-power illumination via a fiber-optic network.

Claims

1. An electrophoresis device comprising:

a plurality of modules comprising a plurality of electrophoretic channels adapted to separate molecules according to attributes of the molecules;

a light source, illuminating each of the modules with light;

a plurality of local processors, each module being connected to a local processor, wherein the local processors collect data from the modules, and the data is determined according to a change in the light source passing through a portion of the module; and

a global processor connected to each of the local processors, the global processor adapted to present data from at least one local processor.

2. The device of claim 1, wherein the modules are asynchronous.

3. The device of claim 1, further comprising a programmable fiber-optic switch for splitting the light among the modules.

4. The device of claim 3, wherein the illumination of each module can be individually controlled by the switch.

5. The device of claim 1, further comprising at least one local host connected to at least one local processor.

6. The device of claim 5, wherein a plurality of local processors are remotely distributed.

7. The device of claim 1, further comprising a programmable fiber-optic switch for splitting the light among the modules, wherein the switch is controlled by the global processor.

8. The device of claim 1, wherein the global processor connected at least one local host, wherein each local host is connected to at least one local processor, the global processor adapted to present data from at least one module.

9. The device of claim 1, wherein the light source is a laser.

10. The device of claim 9, wherein the laser is a multicolor modulated laser.

11. The device of claim 1, further comprising a detection device for determining changes in the light.

12. The device of claim 11, wherein the detection device is a single-photon detection module.

13. An electrophoresis device comprising;

a plurality of modules comprising a plurality of electrophoretic channels adapted to separate molecules according to attributes of the molecules;

a laser source, illuminating each of the modules with laser light;

a programmable fiber-optic switch for splitting the laser light among the modules;

a plurality of local processors, each module being connected to a local processor, wherein the local processors collect data from the modules, and the data is determined according to a change in the laser light passing through a portion of the module; and

a global processor connected to at least a local processor, the global processor adapted to present data from it least one module, and to control the programmable fiber-optic-switch.