Microfluidic devices and systems incorporating integrated optical elements

• US 6,498,353 B2
• Filed: 10/24/2001
• Issued: 12/24/2002
• Est. Priority Date: 02/24/1998
• Status: Expired due to Term

• Current Assignee: Caliper Holdings Incorporated
• Original Assignee: Caliper Holdings Incorporated
• Inventors: Kennedy, Colin B., Nagle, Robert
• Primary Examiner(s): LE, QUE TAN
• Application Number:
  US10/038,525
• Publication Number:
  US 20020092973A1
• Time in Patent Office: 426 Days
• Field of Search: 250/573, 250/216, 250/576, 250/574, 356/246, 356/244, 422/99, 422/104, 422/108, 422/100, 204/603, 204/601, 204/612, 204/297.R
• US Class Current: 250/573
• CPC Class Codes: B01L 2300/0654 Lenses; Optical fibres
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2300/0887 Laminated structure
• CPC Class Codes: B01L 2400/0415 electrical forces, e.g. electrokinetic
• CPC Class Codes: B01L 2400/0487 fluid pressure, pneumatics
• CPC Class Codes: B01L 3/5027 by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip B01L3/5023 takes precedence; micromixers B01F33/30; microreactors for synthesis B01J19/0093; microcapillary devices in general B81B1/00
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: G01N 21/0303 Optical path conditioning in cuvettes, e.g. windows; adapted optical elements or systems; path modifying or adjustment G01N21/031 - G01N21/15 take precedence

Abstract

The present invention provides microfluidic devices and systems that utilize optical detection systems, and where one or more light altering optical elements are integrated into the body structure of the microfluidic device. The resulting devices perform at least a portion of the optical manipulations used in the optical detection method employed.

Claims

1. A method for transmitting energy from or to at least one of two or more intersecting microscale channels disposed in a body structure of a microfluidic device comprising transmitting energy through an energy altering element located proximate to the at least one microscale channel in the body structure of the device, whereby at least a portion of said transmitted energy passing from or to the at least one microscale channel is transmitted through the energy altering element.

2. The method of claim 1 wherein said energy altering element includes a light altering optical element.

3. The method of claim 2, wherein the light altering optical element comprises a lens.

4. The method of claim 3, wherein the lens is selected from a magnifying lens, a demagnifying lens, and a collimating lens.

5. The method of claim 2, wherein the light altering optical element comprises a wavelength selective filter.

6. The method of claim 1 further comprising detecting at least a portion of the energy transmitted through the energy altering element with a detector positioned adjacent to said at least one microscale channel.

7. The method of claim 6 further comprising passing said transmitted energy through a detection window disposed on an exterior portion of the body structure prior to detecting said transmitted energy with said detector.

8. The method of claim 7 further comprising positioning the detector adjacent the detection window prior to detecting said transmitted energy with said detector.

9. The method of claim 8 wherein the detector is a light detector.

10. The method of claim 9 wherein the light detector is selected from a photomultiplier tube and a photodiode.

11. The method of claim 2, wherein the light altering optical element comprises a light altering optical coating disposed on an outer surface of the body structure.

12. A microfluidic device comprising a body structure, two or more intersecting microscale channels disposed in the body structure, and a means for altering light integrated into the body structure adjacent to at least one of the two or more intersecting microscale channels which is capable of transmitting or receiving light passing from or to the at least one microscale channel.

13. The device of claim 12 wherein the light altering means comprises a lens.

14. The device of claim 13, wherein the lens is selected from a magnifying lens, a demagnifying lens, and a collimating lens.

15. The device of claim 12, wherein the light altering means comprises a wavelength selective filter.

16. The device of claim 12 wherein the light altering means comprises an optical coating.

17. The device of claim 16 wherein the optical coating comprises one of wavelength selective filter, a spatial optical filter, and an antireflective coating.

18. A microfluidic device, comprising:

a first planar substrate having at least first and second opposing planar surfaces, and at least one microscale channel being fabricated into the first planar surface of the first substrate;

a second planar substrate having at least first and second opposing planar surfaces, said first planar surface of the second substrate being bonded to the first planar surface of the first substrate; and

a third planar substrate having at least a first planar surface and a second planar surface, the first planar surface of the third substrate being attached to one of the second planar surface of the first substrate or the second planar surface of the second substrate, said third planar substrate including a light altering optical element which is fabricated into the second planar surface of the third substrate.

19. The device of claim 18 wherein the light altering optical element comprises a lens.

20. The device of claim 18, wherein the lens is selected from a magnifying lens, a demagnifying lens, and a collimating lens.

21. The device of claim 18, wherein the light altering optical element comprises a wavelength selective filter.

22. The device of claim 18 wherein the light altering optical element comprises an optical coating.

23. The device of claim 22 wherein the optical coating comprises one of wavelength selective filter, a spatial optical filter, and an antireflective coating.