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Method for sealing and venting a microplate assembly

  • US 6,500,390 B1
  • Filed: 12/13/1999
  • Issued: 12/31/2002
  • Est. Priority Date: 10/17/1996
  • Status: Expired due to Fees
First Claim
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1. A method of sealing a vessel, to prevent independent passage of liquid from said vessel for all possible positions of the vessel, and venting said vessel, to equalize the gas pressure between interior and exterior of the vessel, and wherein said vessel has an opening and an interior volume with a liquid-holding space located therein, said method comprising:

  • mounting a cover over said opening to form a friction-tight impermeable seal between said cover and a surface of said opening;

    forming a vent hole in said interior of said vessel outside said liquid-holding space for all possible positions of said vessel;

    extending a tubular passage from said vent hole through said cover to the exterior of said vessel such that said vent hole communicates with said interior volume outside said liquid with interior volume outside said liquid-holding space and said exterior of said vessel; and

    placing a resilient barrier across said tubular passage to partially cover said vent hole wherein said resilient barrier defines an axial opening which forms a vent when not penetrated by a probe.

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