Multi-ejector system for ejecting biofluids

• US 6,503,454 B1
• Filed: 11/22/2000
• Issued: 01/07/2003
• Est. Priority Date: 11/22/2000
• Status: Expired due to Term

• Current Assignee: Xerox Corporation (Xerox Holdings Corp.)
• Original Assignee: Xerox Corporation (Xerox Holdings Corp.)
• Inventors: Noolandi, Jaan, Bruce, Richard H., Elrod, Scott A., Hadimioglu, Babur B., Horine, David A.
• Primary Examiner(s): Warden, Jill
• Assistant Examiner(s): Gordon, Brian R
• Application Number:
  US09/721,389
• Time in Patent Office: 776 Days
• Field of Search: 422/100, 422/102, 422/99, 436/180, 347/48, 347/51, 347/40, 347/44, 347/68, 347/71, 347/72, 347/50, 347/55, 347/54
• US Class Current: 436/180
• CPC Class Codes: B01L 3/0268 using pulse dispensing or spraying, eg. inkjet type, piezo actuated ejection of droplets from capillaries
• CPC Class Codes: G01N 2035/1039 Micropipettes, e.g. microcapillary tubes
• CPC Class Codes: Y10T 436/2575 Volumetric liquid transfer

Abstract

A multiple-ejector system for printing arrays of biofluids include a tooling plate having a plurality of sets of tooling pins extending outward from the surface of the tooling plate. A printed circuit board is provided having pairs of power connection pins and ground return pins extending from a surface of the circuit board. A plurality of biofluid drop ejection units are provided and include alignment grooves and at least a transducer. Each of the plurality of biofluid drop ejection units are connected to a corresponding one of a set of tooling pins by connection of the tooling pins and alignment grooves. The power connection pins of the pairs are in operational connection with respective transducers and the ground return connection pins of the pairs are in operational connection with a body portion of the drop ejection units. The different drop ejection units will contain different biofluids which are to be emitted onto a substrate. Verification of drop ejection units containing biofluids, may be obtained in one embodiment through the use of an optical scanner. Detection of drops at defined locations provides a verification that validates a properly formed spot is present on a substrate, and is in the correct position.

Claims

1. A multiple ejector system for printing arrays of biofluids, the multiple ejector system comprising:

a tooling plate having a plurality of sets of tooling pins extending outward from a first surface of the tooling plate;

a printed circuit board having at least a power connection extending from a surface of the circuit board;

wherein said printed circuit board is placed on said first surface of the tooling plate; and

a plurality of biofluid drop ejection units, each unit including alignment grooves and a transducer, each of the plurality of biofluid drop ejection units attached to at least a set of the tooling pins by connection to the alignment grooves.

2. The invention according to claim 1 wherein each of said plurality of sets of tooling pins comprises a power connection pin in operational connection with transducers of each associated drop ejection unit.

3. The invention according to claim 2 further including a plurality of ground return pins extending from the surface of the circuit board, and each of the plurality of ground return pins is connected to one of said drop ejection units.

4. The invention according to claim 2 wherein at least one of the tooling pins acts as a ground return.

5. The invention according to claim 3 wherein said power connection pins are in operational connection with respective transducers, and the ground return connection pins are in operational connection with a body portion of the drop ejection units.

6. The invention according to claim 1 wherein the tooling plate includes openings corresponding to the sets of tooling pins such that when the printed circuit board is brought into contact with a back surface of the tooling plate, the tooling pins pass through the tooling plate openings.

7. The invention according to claim 1 wherein the circuit board includes openings corresponding to the tooling pins such that when the tooling plate is brought into contact with a back surface of the circuit board, the tooling pins pass through the circuit board openings.

8. The invention according to claim 1 wherein the biofluid drop ejection unit for ejecting biofluid drops includes:

a biofluid containment area for holding a low volume of biofluid; and

the transducer is integrated with the biofluid containment area as a single disposable unit, the transducer configured to eject drops of biofluid from the biofluid containment area.

9. The invention according to claim 1 wherein the biofluid drop ejection units are acoustic drop ejection units.

10. The invention according to claim 1 wherein the biofluid drop ejection units are piezoelectric drop ejection units.

11. The invention according to claim 5 wherein each of the drop ejection units are piezoelectric drop ejectors including:

the transducer being a piezo actuator;

a reagent cartridge having at least one surface with a first flexible membrane, which is in operative connection with the piezoelectric transducer; and

a nozzle in operative connection with the reagent cartridge and positioned in relationship to the piezoelectric transducer such that action of the piezoelectric transducer causes the biofluid to be emitted through the nozzle as the biofluid drops.

12. The invention according to claim 2 wherein connection of the alignment grooves and tooling pins locates or aligns the drop ejection units to within one-thousandth of an inch of an ideal position.

13. The invention according to claim 1 wherein the multiple ejection system includes more than one hundred drop ejection units.

14. The invention according to claim 1 wherein different drop ejection units of the multiple drop ejection system contain different biofluids.

15. The invention according to claim 1 wherein the plurality of drop ejection units are formed as part of sub-arrays of drop ejector units.

16. The invention according to claim 1 wherein at least one of the tooling plate and tooling pins include drop ejector stops, wherein as the drop ejector moves past the tooling pins the stops act to hold the drop ejector at a predetermined position.