Chemically specific imaging of tissue
First Claim
1. A method for imaging tissue comprising:
- (a) administering to the tissue a deuterated imaging agent;
(b) irradiating the tissue with a source of electromagnetic radiation; and
(c) collecting and analyzing Raman spectra emitted from the tissue.
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Abstract
Disclosed are methods and materials for obtaining spatially resolved images of specific types of tissues. The method for imaging tissue comprises administering to the tissue a deuterated imaging agent and performing spectroscopy, preferably Raman spectroscopy. Electromagnetic radiation, such as a near infrared laser beam, is directed to a tissue of interest. The radiation can be scanned across and within the tissue of interest. When used in combination with a light collection system, it is possible to map out a specific volume of tissue, obtaining information regarding the distribution of specific endogenous chemical species. In some embodiments, specific imaging agents are employed to impart contrast between chemically different types of tissues.
85 Citations
40 Claims
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1. A method for imaging tissue comprising:
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(a) administering to the tissue a deuterated imaging agent;
(b) irradiating the tissue with a source of electromagnetic radiation; and
(c) collecting and analyzing Raman spectra emitted from the tissue. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 39)
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- 18. A diagnostic composition for use with Raman spectroscopic imaging comprising a deuterated imaging agent selected from the group consisting of phospholipids, alkyl esters, alkyl alcohols, and urea, and a pharmaceutically acceptable carrier.
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20. A nonradioactive diagnostic composition for use with Raman spectroscopic imaging comprising a deuterated antibody or fragment thereof.
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21. A method for imaging tissue comprising:
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(a) administering to the tissue a deuterated imaging agent;
(b) irradiating the tissue with a source of electromagnetic radiation; and
(c) collecting and analyzing spectra emitted from the tissue wherein the analyzing comprises determining a surface fractal dimension of a portion of the tissue having an area and a perimeter. - View Dependent Claims (22, 23, 24, 25, 26, 29, 30, 31, 32, 33, 34, 35, 36, 40)
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27. The method of claim wherein 21, the deuterated imaging agent is capable of selectively penetrating the tissue so as to enhance imaging of a target tissue.
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28. The method of claim wherein the deuterated imaging agent comprises an agent selected from the group consisting of water, phospholipids, alkyl esters, alkyl alcohols, fatty acids, urea and pyrrolidones.
- 37. A diagnostic composition for use with Raman spectroscopic imaging consisting essentially of a deuterated imaging agent selected from the group consisting of phospholipids, alkyl esters, alkyl alcohols, and urea, and a pharmaceutically acceptable carrier.
Specification