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Methods and apparatus for detecting polynucleotide hybridization

  • US 6,503,719 B2
  • Filed: 01/22/2001
  • Issued: 01/07/2003
  • Est. Priority Date: 01/15/1999
  • Status: Expired due to Fees
First Claim
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1. A method of detecting a polynucleotide, the method comprising:

  • providing a substrate having at least two assay sites;

    locating a different reference polynucleotide at each assay site;

    depositing substantially equal amounts of a sample polynucleotide at each assay site under conditions conducive to hybridization, wherein at least one of the reference and sample polynucleotides at each assay site is labeled with a luminophore;

    illuminating each assay site with polarized light;

    detecting polarized light transmitted from each assay site; and

    deriving information relating to the sequence of the sample polynucleotide by comparing the extent of polarization of the light emitted from each assay site.

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