Analytical system and method

• US 6,503,757 B1
• Filed: 10/12/2001
• Issued: 01/07/2003
• Est. Priority Date: 08/02/1996
• Status: Expired due to Term

• Current Assignee: Caliper Technologies Corp (Revvity, Inc.)
• Original Assignee: Caliper Technologies Corp (Revvity, Inc.)
• Inventors: Chow, Calvin Y. H.
• Primary Examiner(s): Drodge, Joseph W.
• Application Number:
  US09/976,132
• Publication Number:
  US 20020192112A1
• Time in Patent Office: 452 Days
• Field of Search: 204/269, 204/403, 204/412, 204/600, 204/601, 204/450, 204/451, 422/50, 422/52, 422/54, 422/55, 422/58, 422/63, 422/67, 422/68.1, 422/81, 422/82.05, 422/82.09, 422/88, 422/99-104, 436/43, 436/44, 436/52, 436/170, 436/171, 436/164, 436/165, 436/174, 436/178, 436/186, 436/514, 216/2, 216/56, 137/551, 137/550, 137/826, 137/833
• US Class Current: 436/43
• CPC Class Codes: B01L 2200/027 for microfluidic devices
• CPC Class Codes: B01L 2200/143 Quality control, feedback systems
• CPC Class Codes: B01L 2300/0645 Electrodes
• CPC Class Codes: B01L 2300/0654 Lenses; Optical fibres
• CPC Class Codes: B01L 2400/0415 electrical forces, e.g. electrokinetic
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: B01L 3/50273 characterised by the means or forces applied to move the fluids micropumps F04B19/006, of the membrane type F04B43/043
• CPC Class Codes: B01L 9/527 for microfluidic devices, e.g. used for lab-on-a-chip
• CPC Class Codes: G01N 2035/00326 Analysers with modular structure
• CPC Class Codes: Y10T 436/11 Automated chemical analysis
• CPC Class Codes: Y10T 436/25 including sample preparation
• CPC Class Codes: Y10T 436/2575 Volumetric liquid transfer

Abstract

An analytical or preparatory microfluidic system is disclosed which generally comprises a base unit comprising a first adapter interface array; a first adapter plate selected from a set of a plurality of different adapter plates, wherein the first adapter plate comprises a base unit interface array that is complementary to the first adapter interface array, and a first substrate interface array, wherein the first adapter plate is removably coupled to the base unit such that energy passes from the base unit to the adapter plate through the first adapter and base unit interface arrays. The system further comprises a first microfluidic substrate selected from a set of a plurality of different microfluidic substrates, the first microfluidic substrate comprising a plurality of mesoscale channels disposed therein and a second adapter interface array, wherein the second adapter interface array is complementary to the first substrate interface array. The first microfluidic substrate is mounted against the first adapter plate, and coupled to the adapter plate such that energy passes from the adapter plate to the microfluidic substrate through the second adapter and first substrate interface arrays. Methods of configuring a microfluidic system as described above to perform analytical and biological analyses and preparatory procedures are also disclosed.

Claims

1. A microfluidic system, comprising:

a base unit comprising a first adapter interface array;

a first adapter plate selected from a set of a plurality of different adapter plates, wherein the first adapter plate comprises;

a base unit interface array that is complementary to the first adapter interface array; and

a first substrate interface array; and

wherein the first adapter plate is removably coupled to the base unit whereby energy passes from the base unit to the adapter plate through the first adapter and base unit interface arrays; and

a first microfluidic substrate selected from a set of a plurality of different microfluidic substrates, the first microfluidic substrate comprising

a plurality of mesoscale channels disposed therein;

a second adapter interface array, wherein the second adapter interface array is complementary to the first substrate interface array; and

wherein the first microfluidic substrate is mounted against the first adapter plate, and coupled to the adapter plate such that energy passes from the adapter plate to the microfluidic substrate through the second adapter and first substrate interface arrays.

2. The microfluidic system of claim 1, wherein the energy is pressure energy, light energy, electrical energy or thermal energy.

3. The microfluidic device of claim 1, wherein the energy comprises pressure energy.

4. The mircofluidic system of claim 1, wherein the first microfluidic substrate is removably mounted against the first adapter plate.

5. The microfluidic system of claim 1, further comprising an optical detector positioned adjacent to the first microfluidic substrate to receive a light energy from at least one of the mesoscale channels.

6. The microfluidic system of claim 5, wherein the optical detector is disposed within the base unit.

7. A method of configuring a microfluidic system, comprising:

providing a base unit having a universal interface array;

providing a plurality of adapter plates, each adapter plate comprising a first interface array that is complementary to the universal interface array on the base unit, and a second interface array;

providing a plurality of different microfluidic substrates, each of the plurality of different microfluidic substrates comprising a plurality of mesoscale channels disposed therein, and comprising an adapter interface array which is complementary to a second interface array on at least one of the plurality of different adapter plates;

connecting a first microfluidic substrate to a first adapter plate, wherein the second interface array on the first adapter plate is configured to connect with the adapter interface array on the first microfluidic substrate; and

connecting the first adapter plate to the base unit through the universal interface array on the base unit and the first interface array on the first adapter plate.

8. The method of claim 7, wherein each of the plurality of different microfluidic substrates is mounted against a different adapter plate.

9. The method of claim 8, wherein the step of connecting the first microfluidic substrate to the first adapter plate comprises removably connecting the first mircofluidic substrate to the first adapter plate.

10. The method of claim 8, wherein the first adapter plate comprises a plurality of flow biasing connectors that communicate flow biasing energy from the base unit to the first microfluidic substrate.

11. The method of claim 10, wherein the step of connecting the first microfluidic substrate to the first adapter comprises connecting the flow biasing connectors on the first adapter plate to regions of the first microfluidic substrate to control and manage flow of fluids in the plurality of mesoscale channels.

12. The method of claim 11, wherein the flow biasing energy comprises pressure energy.

13. The method of claim 12, wherein the regions on the first microfluidic substrate comprise fluid reservoirs disposed in fluid communication with the plurality of mesoscale channels.

14. The method of claim 11, wherein the flow biasing energy comprises mechanical energy.

15. The method of claim 11, wherein the flow biasing energy comprises electrical energy.

16. The method of claim 7, wherein one of the universal interface array and the first interface array comprises a plug connector, and the other of the universal interface array and the first interface array is configured to connect to the plug connector.

17. The method of claim 7, wherein each of the plurality of different microfluidic substrates comprise a different network of mesoscale fluid channels disposed therein.