Optical system for multi-part differential particle discrimination and an apparatus using the same

• US 6,507,400 B1
• Filed: 02/19/2000
• Issued: 01/14/2003
• Est. Priority Date: 02/27/1999
• Status: Expired due to Fees

• Current Assignee: Drew Scientific Holdings, Inc.
• Original Assignee: Mwi, Inc. (Escalon Medical Corporation)
• Inventors: Boyd, James R., Pina, Jean-Charles, Gangstead, Mervin L., West, Jerry B., von Behrens, Wieland
• Primary Examiner(s): STAFIRA, MICHAEL PATRICK
• Application Number:
  US09/507,429
• Time in Patent Office: 1,060 Days
• Field of Search: 356/337-343, 356/349, 356/38, 356/73, 356/442, 356/318, 356/72, 356/317, 250/573-575, 250/461.2
• US Class Current: 356/338
• CPC Class Codes: G01N 15/1456 without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
• CPC Class Codes: G01N 15/149 specially adapted for sorting particles, e.g. by their size or optical properties
• CPC Class Codes: G01N 2015/1006 for cytology
• CPC Class Codes: G01N 2015/1019 Associating Coulter-counter and optical flow cytometer [OFC]
• CPC Class Codes: G01N 2015/1486 Counting the particles
• CPC Class Codes: G01N 2021/0346 Capillary cells; Microcells
• CPC Class Codes: G01N 21/05 Flow-through cuvettes G01N21/09 takes precedence; handling fluid samples G01N1/10
• CPC Class Codes: G01N 21/51 inside a container, e.g. in an ampoule G01N21/53 takes precedence

Abstract

The present invention relates to an optical system for an apparatus for multi-part differential particle discrimination to facilitate analysis, classification, and sorting of various fluid components for presentation. The optical system is characterized by one or more of the following: a synchronized illumination beam and flow cell conduit, a flow cell arrangement to control back reflection, and light sensor arrangement to particularly gather a specific range of light scatter, such specific range of light scatter directly corresponding to at least one type of particle capable of being identified by the apparatus.

Claims

1. A particle analyzing apparatus for discriminating particulate element(s) in a fluid flow for purposes of analysis, classification, sorting, and presentation, the apparatus having an illumination source for emitting an illumination beam, the apparatus comprising:

a flow cell having a flow passage through which a fluid flow containing particles can pass, a first exterior face perpendicular to a first axis of the flow cell, and a second exterior face perpendicular to a second axis of the flow cell, wherein the first axis is substantially co-axial with an illumination beam emitted from the illumination source, the flow passage is positioned between the illumination source and the first exterior face, and the first exterior face and the second exterior face are orthogonal; and

a light sensor to receive light from the illumination source via the flow cell, the light sensor being oriented substantially parallel to the second exterior face of the flow cell and displaced a sufficient distance from the second axis in a direction in parallel with the first axis to enable receipt of a prescribed range of light passed by the first exterior face.

2. An apparatus in accordance with claim 1, further comprising light extinguishing means that is adapted to intercept and extinguish at least a portion of any light passed by the second exterior face.

3. An apparatus in accordance with claim 1, further comprising a focusing optical system, positioned between the illumination source and the flow cell, to position a beam waist of an emitted illumination beam within the flow passage.

4. An apparatus in accordance with claim 1, wherein the illumination source is a solid-state laser diode.

5. An apparatus in accordance with claim 1, wherein the illumination source emits an illumination beam having an intensity profile characterized by a substantially central, maximum intensity peak.

6. An apparatus in accordance with claim 5, wherein the flow cell is arranged relative to the illumination source so that a maximum intensity peak of an emitted illumination beam is substantially centered with respect to the flow passage, and such illumination beam intercepts internal edges of the flow cell that define the flow path at points that generate a minimal light scatter.

7. An apparatus in accordance with claim 6, wherein the internal edges intercept the illumination beam at or about intensity nulls of the illumination beam.

8. An apparatus in accordance with claim 1, wherein the flow cell is not orthogonal to an illumination beam emitted by the illumination source.

9. An apparatus in accordance with claim 1, wherein the flow cell is displaced by an angle relative to a direction orthogonal to an illumination beam emitted by the illumination source, and the angle is between 2.5°

-10°

.

10. An apparatus in accordance with claim 9, further comprising a focusing optical system, positioned between the illumination source and the flow cell, to position a beam waist of an emitted illumination beam within the flow passage.

11. An apparatus in accordance with claim 1, further including at least one additional light sensor, positioned substantially orthogonal to an axis of an illumination beam emitted by the illumination source, to receive light from the illumination source via the flow cell.

12. An apparatus in accordance with claim 1, wherein the light sensor is movable and is adapted to move between a first position and a second position, the second position being substantially centered with the second axis.

13. A particle analyzing apparatus for discriminating particulate element(s) in a fluid flow for purposes of analysis, classification, sorting, and presentation, the apparatus comprising:

an illumination source to emit an illumination beam;

a flow cell having a flow passage extending through the flow cell, a rear surface portion that receives an illumination beam emitted by the illumination source, and a forward surface portion that passes light formed from an illumination beam received by the rear surface portion;

a sample supply to introduce a fluid to the flow passage of the flow cell; and

a light sensor to receive at least light passed from the flow cell in response to an interaction with an illumination beam emitted by the illumination source and a fluid supplied to the flow passage of the flow cell,

wherein the flow cell has a first axis, which is substantially co-axial with an axis of an illumination beam emitted from the illumination source, and a second axis, which is orthogonal to the first axis, and

wherein a light receiving surface of the light sensor is substantially parallel to the axis of an illumination beam emitted from the illumination source, and the light sensor is displaced a prescribed distance from the second axis to enable receipt of a prescribed range of light passed by the forward surface portion.

14. An apparatus in accordance with claim 13, further comprising light extinguishing means that is adapted to intercept and to substantially prevent passage of at least a portion of light passed by the flow cell toward the first light sensor.

15. An apparatus in accordance with claim 13, further comprising a focusing optical system, positioned between the illumination source and the flow cell, to form and to position a beam waist of an emitted illumination beam within the flow passage.

16. An apparatus in accordance with claim 13, wherein the illumination source is a solid-state laser diode.

17. An apparatus in accordance with claim 13, wherein the flow cell is arranged relative to the illumination source so that a maximum intensity peak of an illumination beam emitted by the illumination source is substantially centered with respect to the flow passage, and such illumination beam intercepts internal edges of the flow cell, which define the flow path, at points that yield a minimum light scatter.

18. An apparatus in accordance with claim 17, wherein the internal edges intercept the illumination beam at or about intensity nulls of the illumination beam.

19. An apparatus in accordance with claim 13, wherein the flow cell is not orthogonal to an illumination beam emitted by the illumination source.

20. An apparatus in accordance with claim 19, further comprising a focusing optical system, positioned between the illumination source and the flow cell, to form and to position a beam waist of an emitted illumination beam within the flow passage.

21. An apparatus in accordance with claim 13, wherein the flow cell is displaced by an angle relative to a direction orthogonal to an illumination beam emitted by the illumination source, and the angle is between 2.5°

-10°

.

22. An apparatus in accordance with claim 13, further including a second light sensor, positioned substantially orthogonal to an axis of an illumination beam emitted by the illumination source, to receive light from the illumination source via the flow cell.

23. An apparatus in accordance with claim 13, wherein the light sensor is movable to and from the prescribed distance from the second axis, and a second position, the second position being substantially centered with the second axis.

24. A particle analyzing apparatus for discriminating particulate element(s) in a fluid flow for purposes of analysis, classification, sorting, and presentation, the apparatus having an illumination source for emitting an illumination beam along an optical axis of the apparatus, the apparatus comprising:

a flow cell having a flow passage through which a fluid flow containing particles can pass, a first exterior face perpendicular to a first axis of the flow cell, and a second exterior face parallel to the first exterior face, wherein the flow passage is positioned between the illumination source and the first exterior face; and

a light sensor, oriented substantially parallel to the second exterior face of the flow cell and displaced a sufficient distance from the optical axis to enable receipt of a prescribed range of light passed by the first exterior face of the flow cell,

wherein the first axis is substantially co-axial with the optical axis.

25. A method for optically differentiating at least one type of particulate element carried by a fluid filament, the method comprising the steps of:

providing a laser source and a flow cell, the flow cell having a flow passage through which a fluid flow containing particles can pass, a first exterior face perpendicular to a first axis of the flow cell, and a second exterior face that is orthogonal to the first exterior face, wherein the flow passage is positioned between the illumination source and the first exterior face; and

providing a first light sensor, oriented substantially parallel to the second exterior face of the flow cell and displaced a sufficient distance from a centralized, normal axis of the second exterior face in a direction parallel to the first axis to enable receipt of a first prescribed range of light passed by the first exterior face;

providing a second light sensor to receive a second prescribed range of light passed by the flow cell;

emitting a laser beam from a laser source along an optical path and through the flow cell during passage of a fluid filament through the flow passage;

sensing at least a portion of light scatter, using the first light sensor, produced by an interaction between a particulate element, carried by the fluid filament, and the laser beam; and

compare data representative of light scatter sensed by the first light sensor and data representative of light scatter sensed by the second light sensor and, based on such comparison, identify common data representative of light scatter formed independent of the interaction between the particulate element and the laser beam.

26. A particle analyzing apparatus for discriminating particulate element(s) in a fluid flow for purposes of analysis, classification, sorting, and presentation, the apparatus having an illumination source for emitting an illumination beam, the apparatus comprising:

a flow cell having a flow passage through which a fluid flow containing particles can pass, a first exterior face perpendicular to a first axis of the flow cell, and a second exterior face perpendicular to a second axis of the flow cell, wherein the first axis is substantially co-axial with an illumination beam emitted from the illumination source, and the first exterior face and the second exterior face are orthogonal; and

a light sensor to receive light from the illumination source via the flow cell, the light sensor being oriented substantially parallel to the first exterior face of the flow cell and displaced a sufficient distance from the first axis in a direction in parallel with the second axis to enable receipt of a prescribed range of light passed by the second exterior face.

27. An apparatus in accordance with claim 26, further comprising a focusing optical system, positioned between the illumination source and the flow cell, to position a beam waist of an emitted illumination beam within the flow passage.

28. An apparatus in accordance with claim 26, wherein the illumination source is a solid-state laser diode.

29. An apparatus in accordance with claim 26, wherein the illumination source emits an illumination beam having an intensity profile characterized by a substantially central, maximum intensity peak.

30. An apparatus in accordance with claim 29, wherein the flow cell is arranged relative to the illumination source so that a maximum intensity peak of an emitted illumination beam is substantially centered with respect to the flow passage, and such illumination beam intercepts internal edges of the flow cell that define the flow path at points that generate a minimal light scatter.

31. An apparatus in accordance with claim 30, wherein the internal edges intercept the illumination beam at or about intensity nulls of the illumination beam.

32. An apparatus in accordance with claim 26 wherein the flow cell is not orthogonal to an illumination beam emitted by the illumination source.

33. An apparatus in accordance with claim 26, wherein the flow cell is displaced by an angle relative to a direction orthogonal to an illumination beam emitted by the illumination source, and the angle is between 2.5°

-10°

.

34. An apparatus in accordance with claim 33, further comprising a focusing optical system, positioned between the illumination source and the flow cell, to position a beam waist of an emitted illumination beam within the flow passage.

35. An apparatus in accordance with claim 26, further including at least one additional light sensor, positioned substantially orthogonal to an axis of an illumination beam emitted by the illumination source, to receive light from the illumination source via the flow cell.

36. An apparatus in accordance with claim 26, wherein the light sensor is movable and is adapted to move between a first position and a second position, the second position being substantially centered with the second axis.