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Methods for designing exogenous regulatory molecules

  • US 6,511,808 B2
  • Filed: 04/27/2001
  • Issued: 01/28/2003
  • Est. Priority Date: 04/28/2000
  • Status: Expired due to Term
First Claim
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1. A method for designing one or more exogenous regulatory molecules for regulating a gene of interest, the method comprising:

  • (a) providing one or more collections of polynucleotide sequences, each collection comprising a plurality of polynucleotide sequences corresponding to accessible regions of cellular chromatin in a sample;

    (b) identifying one or more sequence elements from the collection, wherein the one or more sequence elements are potential regulatory sequences for the gene of interest; and

    (c) preparing an exogenous regulatory molecule that comprises a DNA binding domain and a functional domain that activates or represses transcription of the gene of interest, wherein said preparing comprises selecting the DNA binding domain, the functional domain or both the DNA binding domain and the functional domain based upon the identified sequence elements.

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