Method and apparatus for magnetic separation of particles

• US 6,514,415 B2
• Filed: 01/30/2001
• Issued: 02/04/2003
• Est. Priority Date: 01/31/2000
• Status: Expired due to Term

• Current Assignee: Dexter Magnetic Technologies, Inc. (TSL Engineered Products LLC)
• Original Assignee: Dexter Magnetic Technologies, Inc. (TSL Engineered Products LLC)
• Inventors: Schilling, Mike, Hatch, Gareth
• Primary Examiner(s): Reifsnyder, David A.
• Application Number:
  US09/774,825
• Publication Number:
  US 20020084225A1
• Time in Patent Office: 735 Days
• Field of Search: 210/222, 210/695, 209/215, 209/223.1, 422/99, 422/101, 422/104, 422/186.01, 485/288.4, 485/305.2, 436/526, 436/809, 436/824
• US Class Current: 210/695
• CPC Class Codes: B01L 3/5085 for multiple samples, e.g. microtitration plates
• CPC Class Codes: B03C 1/01 by addition of magnetic adjuvants
• CPC Class Codes: B03C 1/0332 using permanent magnets
• CPC Class Codes: B03C 1/286 disposed at the inner circumference of a recipient, e.g. magnetic drain bolt
• CPC Class Codes: B03C 1/288 disposed at the outer circumference of a recipient
• CPC Class Codes: B03C 2201/18 Magnetic separation whereby the particles are suspended in a liquid
• CPC Class Codes: B03C 2201/26 for use in medical applications
• CPC Class Codes: G01N 35/0098 involving analyte bound to insoluble magnetic carrier, e.g. using magnetic separation magnetic particles used in immunoassays G01N33/54326; magnetic separation in general B03C
• CPC Class Codes: Y10S 436/809 Multifield plates or multicontainer arrays

Abstract

A method and apparatus for magnetic separation of particles within a container. In one embodiment, a container contains a number of particles and a number of magnetically susceptible particles. A number of magnets are arranged in a plane and is placed close to the container. The magnetic poles of the magnets are arranged in a pattern to apply magnetic fields oriented perpendicular to the plane on the container. The pole pattern provides in consistent separation across the container of the number of magnetically susceptible particles from the rest of the particles.

Claims

1. A magnetic separation apparatus comprising:

a container to contain a plurality of particles and a plurality of magnetically susceptible particles;

a plurality of magnets arranged in a plane proximate the container, such that a plurality of magnetic poles of the plurality of magnets apply magnetic fields oriented perpendicular to the plane to facilitate substantially consistent separation of the plurality of magnetically susceptible particles from the plurality of particles across the container.

2. The apparatus of claim 1, wherein the container comprises a multi-well micro-plate.

3. The apparatus of claim 2, wherein the multi-well micro-plate comprises at least one of a 96-well micro-plate, a 384-well micro-plate, and a 1536-well microplate.

4. The apparatus of claim 1, wherein the container comprises at least one of a tube, a vial, a Petri dish, and a bottle.

5. The apparatus of claim 1, wherein the plurality of magnets comprises a plurality of magnets encased within a protective housing.

6. The apparatus of claim 1, wherein the plurality of magnets comprises at least one of a plurality of permanent magnets and a plurality of electromagnets.

7. The apparatus of claim 1, wherein the plurality of poles of the plurality of magnets comprises a plurality of alternating poles arranged in a repeating pattern.

8. A method for magnetic separation comprising:

providing a container comprising a plurality of particles and a plurality of magnetically susceptible particles;

arranging a plurality of magnets in a plane proximate the container, such that a plurality of magnetic poles of the plurality of magnets apply magnetic fields oriented perpendicular to the plane to substantially consistently separate the plurality of magnetically susceptible particles from the plurality of particles across the container.

9. The method of claim 8, wherein said providing the container comprises providing a multi-well micro-plate.

10. The method of claim 9, wherein said providing the multi-well micro-plate comprises providing at least one of a 96-well micro-plate, a 384-well micro-plate, and a 1536-well micro-plate.

11. The method of claim 8, wherein said providing the container comprises providing at least one of a tube, a Petri dish, and a bottle.

12. The method of claim 8, wherein said arranging the plurality of magnets comprises encasing the arranged plurality of magnets within a protective housing.

13. The method of claim 8, wherein said arranging the plurality of magnets comprises arranging at least one of a plurality of permanent magnets and a plurality of electromagnets.

14. The method of claim 8, wherein arranging the plurality of magnetic poles comprises arranging a plurality of alternating poles in a repeating pattern.