Methods and articles for labeling polymer gels

• US 6,521,111 B1
• Filed: 04/10/2000
• Issued: 02/18/2003
• Est. Priority Date: 04/10/2000
• Status: Expired due to Term

• Current Assignee: Life Technologies Corporation (Thermo Fisher Scientific Incorporated)
• Original Assignee: Invitrogen Corp. (Thermo Fisher Scientific Incorporated)
• Inventors: Bautista, Allen, Amshey, Joseph W.
• Primary Examiner(s): Alexander, Lyle A.
• Assistant Examiner(s): BROWN, JENNINE M
• Application Number:
  US09/546,574
• Time in Patent Office: 1,044 Days
• Field of Search: 204/1, 204/600
• US Class Current: 204/616
• CPC Class Codes: G01N 27/44704 Details; Accessories
• CPC Class Codes: G01N 27/44747 Composition of gel or of carrier mixture

Abstract

The invention provides methods, apparatus and kits for integrally labeling polymeric gels, such as polyacrylamide and agarose gels, with indicia that can serve, inter alia, unambiguously to identify the individual gel, using polymeric film labeled with detectable indicia and coated with materials that bond to the gel matrix as the gel polymerizes.

Claims

1. A method of detectably labeling a polymer gel, comprising:

bonding to said gel, during said gel'"'"'s polymerization, a polymeric film having indicia that are detectable after said film is so bonded to said gel,

wherein said polymeric film has a smaller surface area than said gel.

2. The method of claim 1, wherein said gel is a polyacrylamide gel.

3. The method of claim 2, wherein said polyacrylamide gel is a polymerization product of acrylamide monomer crosslinked with N,N′

-methylenebisacrylamide.

4. The method of claim 1, wherein said gel is an agarose gel.

5. The method of claim 4, wherein said agarose is a natural agarose.

6. The method of claim 1, wherein said polymeric film consists essentially of a polymer, or is a laminate at least one layer of which consists essentially of a polymer, selected from the group consisting of polyester, Mylas, polymethylpentene, polystyrene, polypropylene, polyethylene, styreneacrylonitrile copolymers, polycarbonate, cellulose acetate proprionate, cellulose acetate butyrate, nitrile-acrylonitrilestyrene copolymers, polyacrylate, polyterephthalate, polymethacrylate, and acrylonitrile-butadiene-styrene copolymer.

7. The method of claim 6, where said polymeric film consists essentially of polyester.

8. The method of claim 1, wherein said polymeric film is substantially transparent to visible light.

9. The method of claim 1, wherein said bonding is covalent.

10. The method of claim 1, wherein said bonding is noncovalent, but of sufficient strength to prevent separation of the polymeric film from the gel during ordinary handling.

11. The method of claim 1, wherein said polymeric film bonds directly to said gel.

12. The method of claim 1, wherein said polymeric film bonds to said gel indirectly through a coating disposed between said polymeric film and said gel.

13. The method of claim 12, wherein said coating consists essentially of a hydrophilic resin.

14. The method of claim 13, wherein said hydrophilic resin has ethylenically unsaturated groups.

15. The method of claim 14, wherein said resin is allylglycidylagarose.

16. The method of claim 14, wherein said resin is allylglycidyldextran.

17. The method of claim 12, wherein said coating consists of successive layers, from polymeric film to gel, of vinyl acetate-maleic acid copolymer and dried agarose.

18. The method of claim 1, wherein said indicia are detectable before electrophoresis.

19. The method of claim 1, wherein said indicia are detectable upon epiillumination.

20. The method of claim 1, wherein said indicia are detectable upon transillumination.

21. The method of claim 1, wherein said indicia are capable of fluorescing.

22. The method of claim 1, wherein said indicia are capable of chemiluminescing.

23. The method of claim 1, wherein said indicia contain ligands that are capable of binding detectable reagents.

24. A polymer gel labeled with detectable indicia, comprising:

a polymer gel; and

a polymeric film having detectable indicia, wherein said polymeric film is bonded to said polymer gel, said indicia are detectable with the film so bonded, and said polymeric film has a smaller surface area than said gel.

25. The labeled polymer gel of claim 24, wherein said gel is a polyacrylamide gel.

26. The labeled polymer gel of claim 25, wherein said polyacrylamide is a polymerization product of acrylamide monomer crosslinked with N,N′

-methylenebisacrylamide.

27. The labeled polymer gel of claim 24, wherein said gel is an agarose gel.

28. The labeled polymer gel of claim 27, wherein said agarose is a natural agarose.

29. The labeled polymer gel of claim 24, wherein said polymeric film consists essentially of a polymer, or is a laminate at least one layer of which consists essentially of a polymer, selected from the group consisting of polyester, mylar, polymethylpentene, polystyrene, polypropylene, polyethylene, styreneacrylonitrile copolymers, polycarbonate, cellulose acetate proprionate, cellulose acetate butyrate, nitrile-acrylonitrilestyrene copolymers, polyacrylate, polyterephthalate, polymethacrylate, and acrylonitrile-butadiene-styrene copolymer.

30. The labeled polymer gel of claim 29, where said polymeric film consists essentially of polyester.

31. The labeled polymer gel of claim 24, wherein said polymeric film is substantially transparent to visible light.

32. The labeled polymer gel of claim 24, wherein said polymeric film is covalently bonded to said polymer gel.

33. The labeled polymer gel of claim 24, wherein said polymeric film is bonded noncovalently, but sufficiently strongly to said polymer gel so as to prevent separation of the polymeric film from the gel during ordinary handling.

34. The labeled polymer gel of claim 24, wherein said polymeric film is bonded directly to said gel.

35. The labeled polymer gel of claim 24, wherein said polymeric film is bonded to said gel indirectly through a coating disposed between said polymeric film and said gel.

36. The labeled polymer gel of claim 35, wherein said coating consists essentially of a hydrophilic resin.

37. The labeled polymer gel of claim 36, wherein said hydrophilic resin has ethylenically unsaturated groups.

38. The labeled polymer gel of claim 37, wherein said resin is allylglycidylagarose.

39. The labeled polymer gel of claim 37, wherein said resin is allylglycidyldextran.

40. The labeled polymer gel of claim 35, wherein said coating consists of successive layers, from polymeric film to gel, of vinyl acetate-maleic acid copolymer and dried agarose.

41. The labeled polymer gel of claim 24, wherein said indicia are detectable before electrophoresis.

42. The labeled polymer gel of claim 24, wherein said indicia are detectable upon epiillumination.

43. The labeled polymer gel of claim 24, wherein said indicia are detectable upon transillumination.

44. The labeled polymer gel of claim 24, wherein said indicia are capable of fluorescing.

45. The labeled polymer gel of claim 24, wherein said indicia are capable of chemiluminescing.

46. The labeled polymer gel of claim 24, wherein said indicia contain ligands that are capable of binding detectable reagents.