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Apparatus and methods for correcting for variable velocity in microfluidic systems

  • US 6,524,790 B1
  • Filed: 06/08/1998
  • Issued: 02/25/2003
  • Est. Priority Date: 06/09/1997
  • Status: Expired due to Term
First Claim
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1. A method of determining concentration of a reaction or assay product (Cp) in a microfluidic device, the method comprising:

  • (i) converting a labeled first reactant or assay component having a velocity (Ur) and a label (Lr) and a concentration (Cr), the labeled first reactant or assay component producing a signal (Sas) in a signal detection system, to a reaction or assay product comprising a label Lp, having a velocity (Up), wherein (Ur) does not equal (Up);

    (ii) detecting a resulting change in Sas, which change is dependent on a difference between Up and Ur, wherein the change in Sas correlates to a ratio of Ur to Up or a ratio of Cp to Cr; and

    , (iii) determining Cp from the ratio of Cp to Cr, or Ur to Up.

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