Apparatus and methods for correcting for variable velocity in microfluidic systems
First Claim
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1. A method of determining concentration of a reaction or assay product (Cp) in a microfluidic device, the method comprising:
- (i) converting a labeled first reactant or assay component having a velocity (Ur) and a label (Lr) and a concentration (Cr), the labeled first reactant or assay component producing a signal (Sas) in a signal detection system, to a reaction or assay product comprising a label Lp, having a velocity (Up), wherein (Ur) does not equal (Up);
(ii) detecting a resulting change in Sas, which change is dependent on a difference between Up and Ur, wherein the change in Sas correlates to a ratio of Ur to Up or a ratio of Cp to Cr; and
, (iii) determining Cp from the ratio of Cp to Cr, or Ur to Up.
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Abstract
Electrokinetic devices having a computer for correcting for electrokinetic effects are provided. Methods of correcting for electrokinetic effects by establishing the velocity of reactants and products in a reaction in electrokinetic microfluidic devices are also provided. These microfluidic devices can have substrates with channels, depressions, and/or wells for moving, mixing and monitoring precise amounts of analyte fluids.
206 Citations
50 Claims
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1. A method of determining concentration of a reaction or assay product (Cp) in a microfluidic device, the method comprising:
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(i) converting a labeled first reactant or assay component having a velocity (Ur) and a label (Lr) and a concentration (Cr), the labeled first reactant or assay component producing a signal (Sas) in a signal detection system, to a reaction or assay product comprising a label Lp, having a velocity (Up), wherein (Ur) does not equal (Up);
(ii) detecting a resulting change in Sas, which change is dependent on a difference between Up and Ur, wherein the change in Sas correlates to a ratio of Ur to Up or a ratio of Cp to Cr; and
,(iii) determining Cp from the ratio of Cp to Cr, or Ur to Up. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50)
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Specification