×

Method for DNA- or RNA-sequencing

  • US 6,524,829 B1
  • Filed: 06/29/2001
  • Issued: 02/25/2003
  • Est. Priority Date: 09/30/1998
  • Status: Expired due to Term
First Claim
Patent Images

1. A method for base sequencing of DNA or RNA comprising the following steps:

  • (1) immobilizing DNA or RNA single strands on a planar surface;

    (2) focussing a laser beam on a single, immobilized single strand;

    (3) producing a DNA or RNA complementary strand of said immobilized, focussed single strand by adding a solution containing (i) a mixture of nucleotides of the bases adenine, cytosine, guanine and thymine for producing a DNA complementary strand or a mixture of nucleotides of the bases adenine, cytosine, guanine and uracil for producing a RNA complementary strand and (ii) a polymerase, wherein at least two of the four nucleotides of the bases adenine, cytosine, guanine, and thymine, or at least two of the four nucleotides of the bases adenine, cytosine, guanine, or uracil are partially or fully luminescence-tagged, said step (3) further comprising (a) detecting each insertion of a luminescence-tagged nucleotide into the complementary strand with a single-molecule detector, and (b) deleting the luminescence signal of the luminescence-tagged nucleotide after detection and prior to insertion of the respective next luminescence-tagged nucleotide, thereby base sequencing DNA or RNA.

View all claims
  • 10 Assignments
Timeline View
Assignment View
    ×
    ×