Nucleic acid mediated electron transfer

• US 6,528,266 B2
• Filed: 04/30/2001
• Issued: 03/04/2003
• Est. Priority Date: 12/10/1993
• Status: Expired due to Fees

• Current Assignee: California Institute of Technology
• Original Assignee: California Institute of Technology
• Inventors: Fraser, Scott E., Kayyem, Jon Faiz, Meade, Thomas J.
• Primary Examiner(s): Zitomer, Stephanie W.
• Application Number:
  US09/845,746
• Publication Number:
  US 20010046679A1
• Time in Patent Office: 673 Days
• Field of Search: 435/6, 435/287.2, 536/23.1, 536/24.1
• US Class Current: 435/6.11
• CPC Class Codes: C07H 21/00 Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
• CPC Class Codes: C07H 23/00 Compounds containing boron, silicon, or a metal, e.g. chelates, vitamin B12 esters with inorganic acids C07H11/00; metal salts, see parent compounds
• CPC Class Codes: C12Q 1/6818 involving interaction of two or more labels, e.g. resonant energy transfer
• CPC Class Codes: C12Q 1/6825 Nucleic acid detection involving sensors
• CPC Class Codes: C12Q 2563/113 the label being electroactive, e.g. redox labels
• CPC Class Codes: C12Q 2565/101 Interaction between at least two labels

Abstract

The present invention provides for the selective covalent modification of nucleic acids with redox active moieties such as transition metal complexes. Electron donor and electron acceptor moieties are covalently bound to the ribose-phosphate backbone of a nucleic acid at predetermined positions The resulting complexes represent a series of new derivatives that are bimolecular templates capable of transferring electrons over very large distances at extremely fast rates. These complexes possess unique structural features which enable the use of an entirely new class of bioconductors and photoactive probes.

Claims

1. A composition comprising an electrode comprising a covalently attached single stranded nucleic acid comprising at least one inosine.

3. A composition according to claim 1 or 2 further comprising a second nucleic acid comprising at least one moiety capable of donating or accepting an electron.

4. A composition according to claim 3 wherein said moiety is a covalently attached electron transfer moiety.

5. A composition according to claim 4 wherein said electron transfer moiety is a transition metal complex.

6. A composition according to claim 5 wherein said transition metal complex comprises iron.

7. A composition according to claim 1 or 2 further comprising an electron transfer mediator.

2. A composition comprising an electrode comprising a covalently attached single stranded nucleic acid comprising at least one xanthine.

8. A composition comprising:

a) an electron comprising a covalently attached single stranded nucleic acid comprising at least one inosine; and

b) a second nucleic acid comprising a covalently attached transition metal complex.

9. A composition comprising:

a) an electrode comprising a covalently attached single stranded nucleic acid comprising at least one xanthine;

b) a second nucleic acid comprising a covalently attached transition metal complex.