Method of finding, recording and evaluating object structures
First Claim
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1. A method of acquiring object structures of an object, comprising:
- projecting an illumination pattern onto an object plane where the object is located;
detecting an object structure of the object with a microscope having a light source and a detector;
recording image data corresponding to said object structure with said microscope;
measuring one or more intensity profiles of said object structure during said recording, wherein said intensity profiles are described mathematically by a convolution of said illumination pattern with said object structure; and
cross-correlating said illumination pattern and a shape of said object structure to determine said one or more intensity profiles.
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Abstract
A method of finding, recording and optionally evaluating object structures, especially on slides, preferably of fluorescent object structures such as gene spots. A microscope with a CCD camera, a scanning microscope or a preferably confocal laser scanning microscope can be used for recording and for the rapid and reliable detection of the object structures, with the image data being recorded using an illumination pattern that is projected into the object plane.
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Citations
15 Claims
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1. A method of acquiring object structures of an object, comprising:
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projecting an illumination pattern onto an object plane where the object is located;
detecting an object structure of the object with a microscope having a light source and a detector;
recording image data corresponding to said object structure with said microscope;
measuring one or more intensity profiles of said object structure during said recording, wherein said intensity profiles are described mathematically by a convolution of said illumination pattern with said object structure; and
cross-correlating said illumination pattern and a shape of said object structure to determine said one or more intensity profiles.
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2. A method of acquiring object structures of an object, comprising:
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projecting an illumination pattern onto an object plane where the object is located;
detecting an object structure of the object with a microscope having a light source and a detector;
recording image data corresponding to said object structure with said microscope;
measuring one or more intensity profiles of said object structure during said recording, wherein said intensity profiles are described mathematically by a convolution of said illumination pattern with said object structure; and
determining a position of said object structure.
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3. A method of acquiring object structures of an object, comprising:
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projecting more than one illumination pattern onto an object plane where the object is located; and
detecting an object structure of the object with a microscope having a light source and a detector, wherein at least two illumination sources are used with at least two illumination masks, and wherein an image recording is paralleled using at least two illumination sources. - View Dependent Claims (4, 5, 6)
implementing a real time data processing with the aid of a dedicated hardware device, said hardware device selected from the group consisting of a digital signal processor (DSP), a field programmable gate array (FPGA), and an application specific integrated circuit (ASIC), such that only extracted object information from the object structures needs to be stored on a data storage unit coupled to said microscope.
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7. A method of finding, recording and evaluating fluorescent gene spots on slides, using a microscope with a CCD camera, a scanning microscope or a confocal laser scanning microscope, wherein image data is recorded using an illumination pattern projected into an object plane, and wherein at least two illumination sources are used with identical or different illumination masks.
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8. A method of finding, recording and evaluating fluorescent gene spots on slides, using a microscope with a CCD camera, a scanning microscope or a confocal laser scanning microscope, wherein image data is recorded using an illumination pattern projected into an object plane, and wherein an image recording is paralleled using at least two illumination sources.
- 9. A method of finding, recording and evaluating fluorescent gene spots on slides, using a microscope with a CCD camera, a scanning microscope or a confocal laser scanning microscope, wherein image data is recorded using an illumination pattern projected into an object plane, and wherein intensity profiles measured over the object structures during the recording are described mathematically by convolution of the illumination pattern with the respective object structures.
- 12. A method of finding, recording and evaluating fluorescent gene spots on slides, using a microscope with a CCD camera, a scanning microscope or a confocal laser scanning microscope, wherein image data is recorded using an illumination pattern projected into an object plane, wherein information about the object structures recorded is extracted with the aid of mathematical deconvolution of measured image data with the illumination pattern, and wherein image data is recorded simultaneously with illuminating light of at least two different wavelengths, and can be detected in at least two different detection channels.
- 14. A method of finding, recording and evaluating fluorescent gene spots on slides, using a microscope with a CCD camera, a scanning microscope or a confocal laser scanning microscope, wherein image data is recorded using an illumination pattern projected into an object plane, wherein illumination and detection are carried out simultaneously via at least two illumination and detection masks, and wherein individual illumination and detection masks have different shapes and sizes.
Specification