Microfluidic devices and systems

• US 6,534,013 B1
• Filed: 02/15/2000
• Issued: 03/18/2003
• Est. Priority Date: 08/05/1997
• Status: Expired due to Term

• Current Assignee: Caliper Holdings Incorporated
• Original Assignee: Caliper Holdings Incorporated
• Inventors: Kennedy, Colin B.
• Primary Examiner(s): KIM, SUN U
• Application Number:
  US09/504,301
• Time in Patent Office: 1,127 Days
• Field of Search: 422/55, 422/58, 422/68.1, 422/82.05, 422/99, 422/100, 422/104, 204/601, 204/603, 738/649.1
• US Class Current: 422/503
• CPC Class Codes: B01L 2200/025 Align devices or objects to ensure defined positions relative to each other
• CPC Class Codes: B01L 2200/087 Ergonomic aspects
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2400/0415 electrical forces, e.g. electrokinetic
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: B01L 9/527 for microfluidic devices, e.g. used for lab-on-a-chip
• CPC Class Codes: G01N 27/44791 Microapparatus sample containers with integrated microfluidic structures B01L3/5027

Abstract

The present invention is generally directed to improved microfluidic devices and systems, and particularly to a microfluidic device which comprises a planar body structure, at least a first channel network comprising intersecting channels disposed within the body structure wherein the first channel network is contained within an area of the planar body structure that is less than about 6 cm²; and a holder assembly configured to receive the body structure, the holder assembly having at least one aperture disposed therethrough from an upper surface to a lower surface thereof, and wherein the body structure is fixedly mounted to the holder assembly.

Claims

1. A microfluidic device, comprising:

a planar body structure;

at least a first channel network comprising intersecting channels disposed within the body structure;

wherein the first channel network is contained within an area of the planar body structure that is less than about 6 cm²; and

a holder assembly configured to receive the body structure, the holder assembly having at least one aperture disposed therethrough from an upper surface to a lower surface thereof, and wherein the body structure is fixedly mounted to the holder assembly.

2. The microfluidic device of claim 1, wherein the first channel network is contained within an area of the planar body structure that is less than about 5 cm².

3. The microfluidic device of claim 1, wherein the first channel network is contained within an area of the planar body structure that is less than about 4 cm².

4. The microfluidic device of claim 1, wherein the first channel network is contained within an area of the planar body structure that is less than about 3 cm².

5. The microfluidic device of claim 1, wherein the first channel network is contained within an area of the planar body structure that is less than about 2 cm².

6. The microfluidic device of claim 1, wherein the first channel network is contained within an area of the planar body structure that is less than about 1 cm².

7. The microfluidic device of claim 1, wherein the body structure comprises a square or rectangular shape having dimensions less than from about 2 cm by about 2 cm.

8. The microfluidic device of claim 1, wherein the body structure comprises a square or rectangular shape having dimensions less than from about 1.5 cm by about 1.5 cm.

9. The microfluidic device of claim 1, wherein the body structure comprises a square or rectangular shape having dimensions less than from about 1 cm by about 1 cm.

10. The microfluidic device of claim 1, wherein the body structure comprises a plurality of reservoirs disposed in a surface of the body structure, the plurality of reservoirs being in fluid communication with the at least first channel network.

11. The microfluidic device of claim 10, wherein the plurality of reservoirs are in communication with unintersected termini of the intersecting channels.

12. The microfluidic device of claim 1, wherein:

the body structure comprises upper and lower planar substrate layers;

the intersecting channels of the first channel network are fabricated as a plurality of grooves on a surface of the lower substrate; and

the upper planar substrate layer is overlaid and bonded to the lower planar substrate layer to seal the plurality of grooves to form the first channel network.

13. The microfluidic device of claim 12, wherein at least one of the upper and lower planar substrate layers is selected from a group consisting of glass, quartz, and a polymeric material.

14. The microfluidic device of claim 1, wherein the holder assembly comprises a cavity, the cavity being sized to fixedly receive the body structure.

15. The microfluidic device of claim 1, wherein the holder assembly comprises at least a first alignment structure for aligning the microfluidic device on a detector instrument.

16. The microfluidic device of claim 1, wherein the holder assembly comprises a polymeric holder assembly.