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Cardiac function comprising implantation of embryonic stem cell in which differentiation has been initiated

  • US 6,534,052 B1
  • Filed: 09/05/2000
  • Issued: 03/18/2003
  • Est. Priority Date: 09/05/2000
  • Status: Expired due to Fees
First Claim
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1. A method for improving cardiac function in a mammal after a myocardial infarct, said method comprising the steps of:

  • maintaining a plurality of undifferentiated mammalian embryonic stem cells in vitro in a culture medium containing at least one selected from the group consisting of leukemia inhibitory factor and fibroblast feeder cells;

    subsequently culturing said undifferentiated mammalian embryonic stem cells in vitro in a culture media in the absence of leukemia inhibitory factor and fibroblast feeder cells to yield a cellular inoculum comprising mammalian cells in which differentiation has been initiated;

    introducing said cellular inoculum to at least a portion of the previously infarcted area of the heart tissue; and

    allowing said introduced cellular inoculum to engraft in situ as viable cells situated within the previously infarcted area of the heart tissue, wherein the engraftment results in improved cardiac function in said mammal.

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