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Regulation of endogenous gene expression in cells using zinc finger proteins

  • US 6,534,261 B1
  • Filed: 01/12/1999
  • Issued: 03/18/2003
  • Est. Priority Date: 01/12/1999
  • Status: Expired due to Term
First Claim
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1. A method of inhibiting transcription of an endogenous cellular gene in an isolated cell, the method comprising the steps of:

  • administering to the cell a nucleic acid molecule comprising a polynucleotide sequence which encodes a first zinc finger protein, wherein said first zinc finger protein is engineered, wherein said polynucleotide sequence is operably linked to a promoter, and wherein the nucleic acid molecule expresses the first zinc finger protein in the cell; and

    contacting a first target site in the endogenous cellular gene with the first zinc finger protein, wherein the Kd of the zinc finger protein is less than about 25 nM;

    thereby inhibiting transcription of the endogenous cellular gene by at least about 20%.

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