Cell lysis device
First Claim
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1. A cell lysis apparatus, comprising:
- a substrate formed of a material that can be micromachined;
an insulator, covering at least a portion of the substrate;
a first electrode, formed on said insulator, and having a first area over which the first electrode forms a first sharp edge;
a second electrode, also formed on said insulator, and having a second area, spaced from said first area, said second area forming a second sharp edge which is spaced from and facing said first sharp edge;
a distance between said first and second sharp edges being less than 100 microns; and
a power supply connection, enabling specified voltages to be applied between said first and second electrodes in a way which enables cell lysing.
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Abstract
A micromachined cell lysis device with electrodes that are spaced by less than 10 μm from one another. The cells are attracted to the space between the.electrodes and then lysed.
137 Citations
18 Claims
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1. A cell lysis apparatus, comprising:
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a substrate formed of a material that can be micromachined;
an insulator, covering at least a portion of the substrate;
a first electrode, formed on said insulator, and having a first area over which the first electrode forms a first sharp edge;
a second electrode, also formed on said insulator, and having a second area, spaced from said first area, said second area forming a second sharp edge which is spaced from and facing said first sharp edge;
a distance between said first and second sharp edges being less than 100 microns; and
a power supply connection, enabling specified voltages to be applied between said first and second electrodes in a way which enables cell lysing. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
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10. A method of lysing, comprising:
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providing a first electrode with a first sharp-edged area and providing a second electrode with a second sharp edged area facing said first extended sharp edged area and separated therefrom by 100 microns or less, said area defining a cell holding area therebetween;
first energizing said first and second electrodes with a first voltage less than 50 volts peak to peak and which is effective to attract cells into said area between said first and second electrode dielectrophoretically; and
second energizing said first and second electrodes with a second voltage, greater than said first voltage, and which is effective to lyse said cells. - View Dependent Claims (11, 12)
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13. A cell lysis apparatus, comprising:
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a substrate;
an insulator, covering at least a portion of the substrate;
a first array, comprising;
a first electrode, formed on said insulator, and having a first area over which the electrode forms a first sharp edge;
a second electrode, also formed on said insulator, and having a second area spaced from said first area, said second extended area forming a second sharp edge which is spaced from and facing said first sharp edge;
a distance between said first and second sharp edges being less than 100 microns, and a cell holding area being formed between said first and second sharp edges;
a second array, comprising;
a first electrode, formed on said insulator, and having a first area over which the first electrode forms a first sharp edge;
a second electrode, also formed on said insulator, and having a second area spaced from said first area, said second extended area forming a second sharp edge which is spaced from and facing said first sharp edge;
a distance between said first and second sharp edges being less than 100 microns, and a cell holding area being formed between said first and second sharp edges; and
a power supply connection, enabling specified voltages to be applied between said first and second electrodes of each of said first and second array in a way which first enables attraction of cells and then enables lysing of cells which are attracted. - View Dependent Claims (14, 15, 16, 17, 18)
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Specification