Cross-linked polymer compositions and methods for their use

• US 6,534,591 B2
• Filed: 08/17/2001
• Issued: 03/18/2003
• Est. Priority Date: 12/18/1995
• Status: Expired due to Term

• Current Assignee: Angiodevice International GmbH
• Original Assignee: Cohesion Technologies Inc. (Angiotech Pharmaceuticals Incorporated)
• Inventors: Rhee, Woonza M., Berg, Richard A., DeLustro, Frank A.
• Primary Examiner(s): Nutter, Nathan M.
• Application Number:
  US09/932,536
• Publication Number:
  US 20020013408A1
• Time in Patent Office: 578 Days
• Field of Search: 525/54.1, 525/419, 525/420, 525/425
• US Class Current: 525/54.1
• CPC Class Codes: A61L 2300/232 Monosaccharides, disaccharides, polysaccharides, lipopolysaccharides
• CPC Class Codes: A61L 2300/252 Polypeptides, proteins, e.g. glycoproteins, lipoproteins, cytokines
• CPC Class Codes: A61L 2300/606 Coatings
• CPC Class Codes: A61L 24/043 Mixtures of macromolecular materials
• CPC Class Codes: A61L 24/08 Polysaccharides A61L24/043 takes precedence
• CPC Class Codes: A61L 27/26 Mixtures of macromolecular compounds
• CPC Class Codes: A61L 27/34 Macromolecular materials
• CPC Class Codes: A61L 27/54 Biologically active materials, e.g. therapeutic substances A61L27/227 takes precedence
• CPC Class Codes: A61L 31/041 Mixtures of macromolecular compounds
• CPC Class Codes: A61L 31/16 Biologically active materials, e.g. therapeutic substances A61L31/047 takes precedence
• CPC Class Codes: C08B 37/0075 Heparin; Heparan sulfate; Derivatives thereof, e.g. heparosan; Purification or extraction methods thereof
• CPC Class Codes: C08G 65/329 with organic compounds
• CPC Class Codes: C08H 1/00 Macromolecular products derived from proteins food proteins A23; glue, gelatine C09H
• CPC Class Codes: C08H 1/06 derived from horn, hoofs, hair, skin or leather
• CPC Class Codes: C08L 5/16 Cyclodextrin; Derivatives thereof
• CPC Class Codes: C08L 71/00 Compositions of polyethers obtained by reactions forming an ether link in the main chain of polyacetals C08L59/00; of epoxy resins C08L63/00; of polythioether-ethers C08L81/02; of polyether-sulfones C08L81/06; Compositions of derivatives of such polymers
• CPC Class Codes: C08L 71/02 Polyalkylene oxides
• CPC Class Codes: Y10S 623/90 Stent for heart valve
• CPC Class Codes: Y10S 623/901 Method of manufacturing prosthetic device

Abstract

Crosslinked polymer compositions comprise a first synthetic polymer containing multiple nucleophilic groups covalently bound to a second synthetic polymer containing multiple electrophilic groups. The first synthetic polymer is preferably a synthetic polypeptide or a polyethylene glycol that has been modified to contain multiple nucleophilic groups, such as primary amino (—NH₂) or thiol (—SH) groups. The second synthetic polymer may be a hydrophilic or hydrophobic synthetic polymer which contains, or has been derivatized to contain, two or more electrophilic groups, such as succinimidyl groups. The compositions may further comprise other components, such as naturally occurring polysaccharides or proteins (such as glycosaminoglycans or collagen) and/or biologically active agents. Also disclosed are methods for using the crosslinked polymer compositions to effect adhesion between a first surface and a second surface; to effect tissue augmentation; to prevent the formation of surgical adhesions; and to coat a surface of a synthetic implant.

Claims

1. A crosslinkable composition comprised of:

(a) a first crosslinkable component having m nucleophilic groups, wherein m≧

2;

(b) a second crosslinkable component having n electrophilic groups capable of reaction with the m nucleophilic groups to form covalent bonds, wherein n≧

2 and m+n≧

5,

wherein each of the first and second crosslinkable components is biocompatible, synthetic, and nonimmunogenic, and crosslinking of the composition results in a biocompatible, nonimmunogenic, crosslinked matrix.

2. The composition of claim 1, wherein the m nucleophilic groups are identical.

3. The composition of claim 1, wherein at least two of the m nucleophilic groups are different.

4. The composition of claim 1, wherein the n electrophilic groups are identical.

5. The composition of claim 2, wherein the n electrophilic groups are identical.

6. The composition of claim 3, wherein the n electrophilic groups are identical.

7. The composition of claim 1, wherein at least two of the n electrophilic groups are different.

8. The composition of claim 2, wherein at least two of the n electrophilic groups are different.

9. The composition of claim 3, wherein at least two of the n electrophilic groups are different.

10. The composition of claim 1, wherein the first and second crosslinkable components are non-naturally occurring, synthetic molecules.

11. The composition of claim 1, wherein the m nucleophilic groups are bound to the first crosslinkable component through linking groups.

12. The composition of claim 1, wherein the n electrophilic groups are bound to the second crosslinkable component through linking groups.

13. The composition of claim 1, wherein at least one of the first and second crosslinkable components is comprised of a hydrophilic polymer.

14. The composition of claim 1, wherein one of the first and second crosslinkable components is comprised of a hydrophobic polymer.

15. The composition of claim 1, wherein the m nucleophilic groups are primary amino groups.

16. The composition of claim 15, wherein the first crosslinkable component is C₂-C₆ hydrocarbyl substituted with amino groups.

17. The composition of claim 15, wherein the first crosslinkable component is a secondary or tertiary amine NR₁R₂R₃ wherein R₁ is hydrogen or an amino-substituted lower alkyl group, and R₂ and R₃ are amino-substituted lower alkyl groups.

18. The composition of claim 15, wherein the n electrophilic groups are selected from the group consisting of succinimidyl ester, sulfosuccinimidyl ester, maleimido, epoxy, isocyanato, thioisocyanato, and ethenesulfonyl.

19. The composition of claim 18, wherein the n electrophilic groups are selected from the group consisting of succinimidyl ester and sulfosuccinimidyl ester.

20. The composition of claim 1, wherein the m nucleophilic groups are sulfhydryl groups.

21. The composition of claim 20, wherein the n electrophilic groups are sulfhydryl-reactive groups selected so as to form a thioester, thioether, or disulfide linkage upon reaction with the sulfhydryl groups.

22. The composition of claim 1, wherein n=2.

23. The composition of claim 1, wherein m=2.

24. The composition of claim 1, further including an imaging agent.

25. The composition of claim 1, further comprising a therapeutically effective amount of a biologically active agent selected from the group consisting of enzymes, receptor antagonists, receptor agonists, hormones, growth factors, autogenous bone marrow, antibiotics, antimicrobial agents, antibodies, cells and genes.

26. The composition of claim 25, wherein the biologically active agent reacts with the first crosslinkable component, the second crosslinkable component, or both.

27. A crosslinkable system comprised of

(a) a first crosslinkable component having m nucleophilic groups, wherein m≧

2; and

(b) a second crosslinkable component having n electrophilic groups capable of reaction with the m nucleophilic groups to form covalent bonds, wherein n≧

2 and m+n≧

5,

wherein each crosslinkable component is biocompatible, synthetic, nonimmunogenic, and physically segregated from the other crosslinkable component, and crosslinking of the two components results in a biocompatible, nonimmunogenic, crosslinked matrix.

28. The crosslinkable system of claim 27, wherein the first crosslinkable component is in an aqueous solution.

29. The crosslinkable system of claim 27, wherein the first crosslinkable component is present in a molar excess relative to the second crosslinkable component.

30. The crosslinkable system of claim 27, wherein the second crosslinkable component is present in a molar excess relative to the first crosslinkable component.