Tissue-engineered tubular construct having circumferentially oriented smooth muscle cells
First Claim
1. A muscular, tubular tissue-engineered construct comprising:
- a substantially tubular construct of living mammalian tissue having a first end and a second end, an inner surface and an outer surface;
wherein the first end, the second end, and the inner surface of the construct define a lumen passing through the construct; and
wherein tissue between said inner surface and said outer surface defines a wall of mammalian smooth muscle cells;
wherein said wall comprises said mammalian smooth muscle cells oriented circumferentially about said lumen; and
wherein said mammalian smooth muscle cells in said wall have a cell density of at least 107 cells/cc.
2 Assignments
0 Petitions
Accused Products
Abstract
Improved methods for the production of tissue-engineered constructs, including muscular tissue constructs such as vascular constructs, are disclosed. The methods include the use of improved substrates for cell growth, improved cell culture media for cell growth, and the use of distensible bodies to impart pulsatile stretching force to lumens of constructs during growth. Also disclosed are improved products and methods for making those products, including substrates and cell culture media, for tissue engineering and tissue culture generally. Improved muscular tissue constructs, including vascular constructs, are also disclosed, which may be used in medicine for the repair or replacement of damaged natural structures. In an embodiment, a muscular, tubular tissue-engineered construct is prepared having a wall of mammalian smooth muscle cells oriented circumferentially about a lumen of the construct at a cell density of at least 107 cells/cc.
110 Citations
9 Claims
-
1. A muscular, tubular tissue-engineered construct comprising:
-
a substantially tubular construct of living mammalian tissue having a first end and a second end, an inner surface and an outer surface;
wherein the first end, the second end, and the inner surface of the construct define a lumen passing through the construct; and
wherein tissue between said inner surface and said outer surface defines a wall of mammalian smooth muscle cells;
wherein said wall comprises said mammalian smooth muscle cells oriented circumferentially about said lumen; and
wherein said mammalian smooth muscle cells in said wall have a cell density of at least 107 cells/cc. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
-
Specification
-
- Resources
-
Current AssigneeMassachusetts Institute of Technology
-
Original AssigneeMassachusetts Institute of Technology
-
InventorsNiklason, Laura E., Gao, Jinming, Langer, Robert S.
-
Primary Examiner(s)Naff, David M.
-
Assistant Examiner(s)Ware, Deborah K.
-
Application NumberUS09/109,427Time in Patent Office1,727 DaysField of Search435/287.1, 435/366, 435/371, 435/325, 435/363, 435/377, 435/395, 424/395, 424/422, 424/93.7, 424/423, 424/548, 424/569, 424/572US Class Current424/423CPC Class CodesA61L 27/507 for artificial blood vessel...C12M 21/08 for producing artificial ti...C12M 23/06 Tubular C12M23/08, C12M23/1...C12M 23/26 flexible flexible container...C12M 25/14 Scaffolds; Matrices in gene...C12M 29/14 Pressurized fluidC12M 35/04 Mechanical means, e.g. soni...C12N 2500/20 Transition metalsC12N 2500/32 Amino acidsC12N 2503/04 Screening or testing on art...C12N 2509/00 Methods for the dissociatio...C12N 2533/40 Polyhydroxyacids, e.g. poly...C12N 5/0068 General culture methods usi...C12N 5/0691 Vascular smooth muscle cell...
