Tissue-engineered tubular construct having circumferentially oriented smooth muscle cells
First Claim
1. A muscular, tubular tissue-engineered construct comprising:
- a substantially tubular construct of living mammalian tissue having a first end and a second end, an inner surface and an outer surface;
wherein the first end, the second end, and the inner surface of the construct define a lumen passing through the construct; and
wherein tissue between said inner surface and said outer surface defines a wall of mammalian smooth muscle cells;
wherein said wall comprises said mammalian smooth muscle cells oriented circumferentially about said lumen; and
wherein said mammalian smooth muscle cells in said wall have a cell density of at least 107 cells/cc.
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Accused Products
Abstract
Improved methods for the production of tissue-engineered constructs, including muscular tissue constructs such as vascular constructs, are disclosed. The methods include the use of improved substrates for cell growth, improved cell culture media for cell growth, and the use of distensible bodies to impart pulsatile stretching force to lumens of constructs during growth. Also disclosed are improved products and methods for making those products, including substrates and cell culture media, for tissue engineering and tissue culture generally. Improved muscular tissue constructs, including vascular constructs, are also disclosed, which may be used in medicine for the repair or replacement of damaged natural structures. In an embodiment, a muscular, tubular tissue-engineered construct is prepared having a wall of mammalian smooth muscle cells oriented circumferentially about a lumen of the construct at a cell density of at least 107 cells/cc.
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Citations
9 Claims
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1. A muscular, tubular tissue-engineered construct comprising:
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a substantially tubular construct of living mammalian tissue having a first end and a second end, an inner surface and an outer surface;
wherein the first end, the second end, and the inner surface of the construct define a lumen passing through the construct; and
wherein tissue between said inner surface and said outer surface defines a wall of mammalian smooth muscle cells;
wherein said wall comprises said mammalian smooth muscle cells oriented circumferentially about said lumen; and
wherein said mammalian smooth muscle cells in said wall have a cell density of at least 107 cells/cc. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
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Specification