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First Claim
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1. A method for creating a peptide and/or protein database comprising the steps of:
- providing a first source of peptides and/or proteins and providing a second source of peptides and/or proteins wherein the first source and the second source are from different tissues, cells, organelles or fractions of each;
generating a 2-D separation of peptides and/or proteins of a first source;
generating a 2-D separation of peptides and/or proteins of a second source;
generating an electronic image of the 2-D separation of peptides and/or proteins of the first source;
generating an electronic image of the 2-D separation of peptides and/or proteins of the second source;
warping one of the electronic images of the 2-D separation of peptides and/or proteins to the other image;
determining peptides and/or proteins common to both the sources after said warping one of the electronic images by analyzing the two 2-D separations of peptides and/or proteins of the first and second sources;
characterizing a physical, chemical or biological property of the peptides and/or proteins previously determined to be common by a technique other than by the 2-D separation of peptides and/or proteins to establish protein identity, thereby confirming commonality of at least a portion of the peptides and/or proteins common to both of the 2-D separations of peptides and/or proteins;
after said confirming, recording in a peptide and/or protein database all peptides and/or said proteins common to both sources as being the same in response to positive confirmation of the portion of the peptides and/or proteins common to both 2-D separations of peptides and/or proteins, thereby generating a database or part of a database of peptides and/or proteins common to both sources;
analyzing peptides and/or proteins not common to both 2-D separations; and
recording in the peptides and/or proteins database results of said analyzing the peptides and/or proteins not common to both 2-D separations, thereby generating a database or part of a database of peptides and/or proteins in one source and not in another source or in a significantly different amount in another source, wherein said proteins and/or peptides not common to both 2-D separations or in a significantly different amount in another source distinguishes one source from another source.
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Abstract
Data acquisition and cataloging are used to classify polypeptides into a reference index or database. The database can be used to identify previously unidentified samples. New polypeptides are characterized and added to the database.
118 Citations
19 Claims
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1. A method for creating a peptide and/or protein database comprising the steps of:
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providing a first source of peptides and/or proteins and providing a second source of peptides and/or proteins wherein the first source and the second source are from different tissues, cells, organelles or fractions of each;
generating a 2-D separation of peptides and/or proteins of a first source;
generating a 2-D separation of peptides and/or proteins of a second source;
generating an electronic image of the 2-D separation of peptides and/or proteins of the first source;
generating an electronic image of the 2-D separation of peptides and/or proteins of the second source;
warping one of the electronic images of the 2-D separation of peptides and/or proteins to the other image;
determining peptides and/or proteins common to both the sources after said warping one of the electronic images by analyzing the two 2-D separations of peptides and/or proteins of the first and second sources;
characterizing a physical, chemical or biological property of the peptides and/or proteins previously determined to be common by a technique other than by the 2-D separation of peptides and/or proteins to establish protein identity, thereby confirming commonality of at least a portion of the peptides and/or proteins common to both of the 2-D separations of peptides and/or proteins;
after said confirming, recording in a peptide and/or protein database all peptides and/or said proteins common to both sources as being the same in response to positive confirmation of the portion of the peptides and/or proteins common to both 2-D separations of peptides and/or proteins, thereby generating a database or part of a database of peptides and/or proteins common to both sources;
analyzing peptides and/or proteins not common to both 2-D separations; and
recording in the peptides and/or proteins database results of said analyzing the peptides and/or proteins not common to both 2-D separations, thereby generating a database or part of a database of peptides and/or proteins in one source and not in another source or in a significantly different amount in another source, wherein said proteins and/or peptides not common to both 2-D separations or in a significantly different amount in another source distinguishes one source from another source. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11)
excising several of the aligned spots common to both 2-D electrophoresis gels from the gels; and
subjecting the excised spots to mass spectrometry analysis.
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6. The method for creating a peptide and/or protein database as set forth in claim 4, wherein said step of analyzing peptide and/or protein spots not common to both 2-D separations comprises the steps of:
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excising plural spots not common to both 2-D electrophoresis gels from the gels; and
subjecting the excised spots to mass spectrometry analysis.
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7. The method of claim 1, wherein said first source and said second source are two different tissues.
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8. The method of claim 1, wherein said first source and said second source are samples obtained from two individuals of a population.
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9. The method of claim 7, wherein said tissues are from one individual or from genetically identical individuals.
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10. The method of claim 1, wherein said first source and said second source are two different cell types.
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11. The method of claim 1, wherein said first source and said second source are two different organelles.
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12. A method for creating a peptide and/or protein database comprising the steps of:
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providing a first source of peptides and/or proteins and providing a second source of peptides and/or proteins wherein the second source is the same as the first source except wherein said second source comprises exposure of tissues, cells, organelles or fractions of each to varying conditions selected from the group consisting of environmental, chemical, disease and aging;
generating a 2-D separation of peptides and/or proteins of a first source;
generating a 2-D separation of peptides and/or proteins of a second source;
generating an electronic image of the 2-D separation of peptides and/or proteins of the first source;
generating an electronic image of the 2-D separation of peptides and/or proteins of the second source;
warping one of the electronic images of the 2-D separation of peptides and/or proteins to the other image;
determining peptides and/or proteins common to both the sources after said warping one of the electronic images by analyzing the two 2-D separations of peptides and/or proteins of the first and second sources;
characterizing a physical, chemical or biological property of the peptides and/or proteins previously determined to be common by a technique other than by the 2-D separation of peptides and/or proteins to establish protein identity, thereby confirming commonality of at least a portion of the peptides and/or proteins common to both of the 2-D separations of peptides and/or proteins;
after said confirming, recording in a peptide and/or protein database all peptides and/or said proteins common to both sources as being the same in response to positive confirmation of the portion of the peptides and/or proteins common to both 2-D separations of peptides and/or proteins, thereby generating a database or part of a database of peptides and/or proteins common to both sources;
analyzing peptides and/or proteins not common to both 2-D separations; and
recording in the peptides and/or proteins database results of said analyzing the peptides and/or proteins not common to both 2-D separations, thereby generating a database or part of a database of peptides and/or proteins in one source and not in another source or in a significantly different amount in another source, wherein said proteins and/or peptides not common to both 2-D separations or in a significantly different amount in another source distinguishes one source from another source. - View Dependent Claims (13, 14, 15, 16, 17, 18, 19)
excising several of the aligned spots common to both 2-D electrophoresis gels from the gels; and
subjecting the excised spots to mass spectrometry analysis.
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17. The method for creating a peptide and/or protein database as set forth in claim 15, wherein said step of analyzing peptide and/or protein spots not common to both 2-D separations comprises the steps of:
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excising plural spots not common to both 2-D electrophoresis gels from the gels; and
subjecting the excised spots to mass spectrometry analysis.
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18. The method of claim 12, wherein said first source and said second source are samples obtained from two individuals of a population.
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19. The method of claim 12, wherein said tissues are from one individual or from genetically identical individuals.
Specification