Detection of target analytes using particles and electrodes

• US 6,541,617 B1
• Filed: 10/27/1999
• Issued: 04/01/2003
• Est. Priority Date: 10/27/1998
• Status: Expired due to Term

• Current Assignee: Roche Molecular Systems Inc (Roche Holding AG)
• Original Assignee: Clinical Micro Sensors Inc. (Roche Holding AG)
• Inventors: Bamdad, Cynthia C., Mucic, Robert C.
• Primary Examiner(s): Whisenant, Ethan
• Assistant Examiner(s): LU, FRANK WEI MIN
• Application Number:
  US09/428,155
• Time in Patent Office: 1,252 Days
• Field of Search: 435/6, 435/91.1, 435/91.2, 436/94, 436/23.1, 436/24.3, 436/24.33, 436/25.3
• US Class Current: 536/23.1
• CPC Class Codes: B01J 13/00 Colloid chemistry, e.g. the production of colloidal materials or their solutions, not otherwise provided for; Making microcapsules or microballoons
• CPC Class Codes: B82Y 15/00 Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
• CPC Class Codes: B82Y 30/00 Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: C12Q 1/6825 Nucleic acid detection involving sensors
• CPC Class Codes: C12Q 2525/197 incorporating a spacer/coupling moiety
• CPC Class Codes: C12Q 2525/313 Branched oligonucleotides
• CPC Class Codes: C12Q 2563/113 the label being electroactive, e.g. redox labels
• CPC Class Codes: C12Q 2565/101 Interaction between at least two labels
• CPC Class Codes: C12Q 2565/1015 labels being on the same oligonucleotide
• CPC Class Codes: G01N 27/3275 Sensing specific biomolecules, e.g. nucleic acid strands, based on an electrode surface reaction
• CPC Class Codes: G01N 27/3277 being a redox reaction, e.g. detection by cyclic voltammetry voltammetry per se G01N27/42, G01N27/48
• CPC Class Codes: G01N 27/48 using polarography, i.e. measuring changes in current under a slowly-varying voltage
• CPC Class Codes: Y10S 977/773 Nanoparticle, i.e. structure having three dimensions of 100 nm or less
• CPC Class Codes: Y10S 977/92 Detection of biochemical
• CPC Class Codes: Y10T 436/143333 Saccharide [e.g., DNA, etc.]

Abstract

The invention relates to the use of particles comprising binding ligands and electron transfer moieties (ETMs). Upon binding of a target analyte, a particle and a reporter composition are associated and transported to an electrode surface. The ETMs are then detected, allowing the presence or absence of the target analyte to be determined.

Claims

1. A colloid particle comprisingt at least one covalently attached electron transfer moiety (ETM).

2. A colloid particle according to claim 1 colloid further comprising a self-assembled monolayer (SAM).

3. A colloid particle according to claim 1 or 2 wherein said colloid particle further comprises at least one covalently attached capture binding ligand.

7. A colloid particle according to claim 3 wherein said capture binding ligand is a nucleic acid.

10. A colloid particle according to claim 1, 5, 6, 7, 8 or 9 wherein said ETM is a transition metal complex.

11. A colloid particle according to claim 10 wherein said transition metal complex is ferrocene.

12. A colloid particle according to claim 2, ,6, 8 or 9 wherein said SAM comprises alkanes.

13. A colloid particle according to claim 1, 5, 6, 7, 8 or 9 wherein said ETM is an organic ETM.

4. A colloid particle comprising a covalently attached conductive oligomer.

5. A colloid particle according to claim 4 further comprising at least one ETM.

6. A colloid particle comprising:

a) a SAM;

b) a capture probe;

c) an amplification sequence; and

d) a label probe hybridized to said amplification sequence, wherein said label probe comprises at least one covalently attached ETM.

8. A colloid particle comprising:

a) at least one ETM;

b) a self-assembled monolayer (SAM); and

c) at least one covalently attached nucleic acid.

9. A colloid particle comprising:

a) a SAM;

b) a first nucleic acid;

c) a second nucleic acid; and

d) a label probe hybridized to said first nucleic acid, wherein said label probe comprises at least one covalently attached ETM.