Arrangement for optically reading out the information from substrates having a multiplicity of individual samples

• US 6,542,241 B1
• Filed: 03/03/2000
• Issued: 04/01/2003
• Est. Priority Date: 03/25/1999
• Status: Expired due to Fees

• Current Assignee: Jena-Optronik GmbH (Airbus SE)
• Original Assignee: Jena-Optronik GmbH (Airbus SE)
• Inventors: Reiland, Werner, Thorwirth, Guenter
• Primary Examiner(s): Rosenberger, Richard A.
• Application Number:
  US09/518,295
• Time in Patent Office: 1,124 Days
• Field of Search: 356/436, 356/440
• US Class Current: 356/436
• CPC Class Codes: B01J 2219/00605 the compounds being directly bound or immobilised to solid supports
• CPC Class Codes: B01J 2219/00612 the surface being inorganic
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: B01J 2219/00707 separated from the reactor apparatus
• CPC Class Codes: G01N 21/31 Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry G01N21/72 takes precedence
• CPC Class Codes: G01N 21/6452 Individual samples arranged in a regular 2D-array, e.g. multiwell plates

Abstract

An arrangement is disclosed for optically reading out the information from substrates having a multiplicity of individual samples, in particular for analyzing chemical and biological sample carriers. The arrangement presents a new possibility for optically reading out the information from matrix-type substrates having a multiplicity of individual samples which allows a fast read-out of a radiation which is influenced by the individual samples with a high degree of sensitivity. This possibility is achieved by the fact that, in the case of optically reading a matrix-type substrate having a multiplicity of metrically ordered pixels, the receiver that is provided is an individual receiver which has high sensitivity and a uniform receiver area, an electrooptical matrix and also an imaging optical system are present, each substrate pixel being assigned to a matrix pixel region by the imaging optical system, and the matrix can be driven in such a way that matrix regions which allow exclusively the feeding of radiation from a substrate pixel to the receiver can be switched separately. Radiation quantities from in each case at least one substrate pixel successively impinging on the receiver over a suitably chosen time interval. The result is that it is possible to evaluate a series of measured radiation quantities from selected sequences of substrate pixels at the output of the receiver.

Claims

1. An arrangement for optical reading out the information from a matrix-type substrate having a multiplicity of individual samples, which represent metrically ordered pixels on the substrate and emit a radiation which is characteristically influenced by an individual sample substance, comprising:

a receiver;

a transfer optical arrangement for separately transferring the radiation emitted by individual substrate pixels to said receiver;

said receiver being a single receiver which has a high sensitivity and a uniform receiver area and being able to take up the radiation from each substrate pixel;

said transfer optical arrangement having an electro-optical matrix, which has the function of a variable light valve for separately transferring radiation from each substrate pixel to the receiver and being arranged in such a way that each substrate pixel being imaged in an optical one-to-one-assigned manner to a matrix region comprising at least one matrix pixel; and

said matrix being able to be driven in such a way that matrix regions of such a size which allow the feeding of radiation from only one substrate pixel to the receiver can be switched separately, radiation quantities from in each case at least one substrate pixel successively impinging on the receiver over a suitable chosen time interval, so that it is possible to evaluate a series of measured radiation quantities from selected sequences of substrate pixels at the output of the receiver.

2. The arrangement as claimed in claim 1, wherein intensive illumination is provided for generating the radiation which is characteristically influenced by the substrate pixels.

3. The arrangement as claimed in claim 2, wherein the radiation which is characteristically influenced by the substrate pixels is a luminescent radiation generated by the illumination.

4. The arrangement as claimed in claim 3, wherein the radiation which is characteristically influenced by the substrate pixels is a fluorescent radiation.

5. The arrangement as claimed in claim 2, wherein, for generating the radiation which is characteristically influenced by the substrate pixels, provision is made of illumination for reading out the transmissivity or reflectivity of the substrate pixels.

6. The arrangement as claimed in claim 1, wherein, for generating the characteristically influenced radiation from the substrate pixels, provision is made of an illumination device having a parallel pencil of rays which, with optical components arranged downstream being taken into account, is suitable for the large-area uniform illumination of all the substrate pixels.

7. The arrangement as claimed in claim 1, wherein for generating the radiation which is characteristically influenced by the substrate pixels, provision is made of a chemical reaction by contact of the substrate pixels with a surrounding medium.

8. The arrangement as claimed in claim 7, wherein for generating the radiation which is characteristically influenced by the substrate pixels, contact of the substrate pixels with a liquid is provided.

9. The arrangement as claimed in claim 1, wherein the transfer optical arrangement is arranged between the substrate and the receiver, it being possible for the characteristic radiation from each substrate pixel to be detected individually on the receiver by assigned matrix regions of the electrooptical matrix being switched to be transparent.

10. The arrangement as claimed in claim 9, wherein each substrate pixel is imaged by the imaging optical system on a respective matrix pixel, it being possible in each case for the characteristic radiation from the assigned substrate pixel to be detected on the receiver by an arbitrary matrix pixel being switched to be transparent.

11. The arrangement as claimed in claim 9, wherein each substrate pixel is imaged by means of the imaging optical system on a respective group of matrix pixels which is geometrically similar to the substrate pixels, it being possible in each case for the characteristic radiation from the assigned substrate pixel to be detected on the receiver by such a group of matrix pixels being switched to be transparent.

12. The arrangement as claimed in claim 6, wherein the transfer optical arrangement is arranged upstream of the substrate in the parallel pencil of rays from the illumination device, the matrix pixels of the electrooptical matrix being illuminated uniformly by the illumination device and it being possible for the substrate pixels to be illuminated individually with the illumination light by activation of defined matrix regions of the electrooptical matrix, and the substrate being connected to the receiver in such a way that the radiation coming from any arbitrary substrate pixel can be taken up by the receiver essentially without any losses.

13. The arrangement as claimed in claim 12, wherein a respective matrix pixel is imaged by the imaging optical system on a substrate pixel, the assigned substrate pixel, for generating the characteristic radiation, being illuminated by activation of the respective matrix pixel.

14. The arrangement as claimed in claim 12, wherein a respective group of matrix pixels which is geometrically similar to the substrate pixels is imaged by the imaging optical system on a substrate pixel, the assigned substrate pixel, for generating the characteristic radiation, being illuminated by activation of the respective group of matrix pixels.

15. The arrangement as claimed in claim 9, wherein the electrooptical matrix is a liquid crystal matrix having high contrast and short switching times.

16. The arrangement as claimed in claim 15, wherein the electrooptical matrix is a ferroelectric liquid crystal matrix having bistable switching states.

17. The arrangement as claimed in claim 9, wherein the imaging optical system is a fiber optical arrangement preferably a fiber plate, there being a light-guiding connection between the substrate pixels and the assigned matrix pixels as a result of direct contact of the fiber optical arrangement with the substrate, on the one hand, and the electrooptical matrix, on the other hand.

18. The arrangement as claimed in claim 1, wherein for the purpose of optical data reduction, the electrooptical matrix can be activated in suitably chosen matrix regions, with the result that radiation quantities from a plurality of selected substrate pixels can be detected simultaneously on the receiver and are combined to form a receiver measured value, it being possible to compare measured values that have been obtained in this way with radiation values of specimen substrates which have been measured under the same preconditions.

19. The arrangement as claimed in claim 3, wherein the illumination device contains an incoherent light source and a narrowband excitation filter tuned to the excitation wavelength of the luminescent material of the substrate pixels.

20. The arrangement as claimed in claim 19, wherein the excitation filter can be exchanged for adaptation to different luminescent materials of the substrate pixels.

21. The arrangement as claimed in claim 19, wherein the illumination device has a relatively broadband powerful light source.

22. The arrangement as claimed in claim 21, wherein the light source is a halogen lamp.

23. The arrangement as claimed in claim 21, wherein the light source is a powerful light-emitting diode.

24. The arrangement as claimed in claim 3, wherein the illumination device contains a coherent light source which is provided with beam-expanding optics and, with regard to the wavelength, is adapted to the excitation wavelength of the luminescent materials of the substrate pixels.

25. The arrangement as claimed in claim 6, wherein the illumination device is designed in a transmitted-light bright-field configuration.

26. The arrangement as claimed in claim 6, wherein the illumination device is designed in a transmitted-light dark-field configuration.

27. The arrangement as claimed in claim 26, wherein a mirror is arranged in the light path of the parallel pencil of rays which transmissively passes through the substrate, the reflected light of which mirror is provided as an additional reflected-light dark-field light pencil with regard to the substrate.

28. The arrangement as claimed in claim 6, wherein the illumination device is designed in a reflected-light dark-field configuration.

29. The arrangement as claimed in claim 12, wherein the illumination device is designed in a reflected-light dark-field configuration, the activation of the matrix pixels resulting in a change to their reflectivity.

30. The arrangement as claimed in claim 3, wherein a rejection filter for eliminating the illumination light is present in the beam path upstream of the receiver.

31. The arrangement as claimed in claim 1, wherein the receiver is a secondary electron multiplier.

32. The arrangement as claimed in claim 1, wherein optical elements which concentrate the characteristically influenced radiation from all the substrate pixels onto the active light-sensitive area of the receiver are provided upstream of the receiver.

33. The arrangement as claimed in claim 32, wherein a converging optical arrangement is arranged upstream of the receiver and is provided for the purpose of imaging the characteristically influenced radiation from each substrate pixel on the active light-sensitive area of the receiver.

34. The arrangement as claimed in claim 32, wherein a radiation-concentrating element is arranged upstream of the receiver.

35. The arrangement as claimed in claim 34, wherein the radiation-concentrating element is a cross-section converter, in the form of a truncated glass cone.

36. The arrangement as claimed in claim 1, wherein an electromechanical actuating unit is provided for regularly readjusting the transfer optical arrangement relative to the substrate.

37. The arrangement as claimed in claim 36, wherein the substrate can be displaced, by the actuating unit, relative to the entire transfer optical arrangement in two directions of an orthogonal plane with respect to the optical axis and can be rotated at least about one axis.

38. The arrangement as claimed in claim 36, wherein the electrooptical matrix can be displaced, by the actuating unit, relative to the imaging optical system and the substrate in two directions of an orthogonal plane with respect to the optical axis and can be rotated at least about one axis.

39. The arrangement as claimed in claim 37 or 38, wherein the mechanical actuating unit that is present is a piezoelectric x, y, Φ

actuator whose driving is oriented, by regulation, to maximizing the luminescent light efficiency for defined substrate pixels, in particular edge pixels, of a specimen substrate.