Process for stimulating microbial activity in a hydrocarbon-bearing, subterranean formation

• US 6,543,535 B2
• Filed: 02/20/2001
• Issued: 04/08/2003
• Est. Priority Date: 03/15/2000
• Status: Expired due to Term

• Current Assignee: Exxonmobil Upstream Research Company (Exxon Mobil Corporation)
• Original Assignee: Exxonmobil Upstream Research Company (Exxon Mobil Corporation)
• Inventors: Hinton, Stephen M., Converse, David R., Sowlay, Mohankumar R., Hieshima, Glenn B., Barnum, Robert S.
• Primary Examiner(s): Neuder, William
• Assistant Examiner(s): Walker, Zakiya
• Application Number:
  US09/789,019
• Publication Number:
  US 20010045279A1
• Time in Patent Office: 777 Days
• Field of Search: 166/246, 166/400-403, 166/268, 405/128.45, 405/128.7, 210/747, 435/262.5, 435/167
• US Class Current: 166/246
• CPC Class Codes: C09K 8/58 Compositions for enhanced recovery methods for obtaining hydrocarbons, i.e. for improving the mobility of the oil, e.g. displacing fluids
• CPC Class Codes: C09K 8/582 characterised by the use of bacteria
• CPC Class Codes: C09K 8/905 Biopolymers
• CPC Class Codes: C12P 39/00 Processes involving microorganisms of different genera in the same process, simultaneously
• CPC Class Codes: C12P 5/023 Methane
• CPC Class Codes: Y02E 50/30 Fuel from waste, e.g. synthetic alcohol or diesel

Abstract

A process is disclosed for stimulating the activity of microbial consortia in a subterranean formation to convert hydrocarbons to methane, which can be produced. Fluid and rock of the formation are analyzed. The presence of microbial consortia is determined and a characterization made (preferably genetic) of at least one microorganism of the consortia, at least one being a methanogenic microorganism. The characterization is compared with at least one known characterization derived from a known microorganism having one or more known physiological and ecological characteristics. This information, together with the information obtained from the analysis of the fluid and rock, is used to determine an ecological environment that promotes in situ microbial degradation of formation hydrocarbons and promotes microbial generation of methane by at least one methanogenic microorganism of the consortia. This information is then used as the basis for modifying the formation environment to produce methane.

Claims

1. A process of stimulating the activity of microbial consortia in a hydrocarbon-bearing, subterranean formation to convert the hydrocarbons to methane, comprising the acts of:

(a) analyzing one or more components of the formation to determine characteristics of the formation environment;

(b) detecting the presence of microbial consortia within the formation;

(c) determining one or more characterizations of one or more microorganisms of the consortia, at least one of the characterizations being of at least one methanogenic microorganism, and comparing the one or more characterizations with at least one known characterization derived from at least one known microorganism having one or more known physiological and ecological characteristics;

(d) using information obtained from acts (a) and (c) for determining an ecological environment that promotes in situ microbial degradation of hydrocarbons and promotes microbial generation of methane by at least one methanogenic microorganism of the consortia; and

(e) modifying the formation environment based on the determinations of act (d) to stimulate microbial conversion of hydrocarbons to methane.

2. The process of claim 1 further comprises prior to act (b) the additional act of introducing one or more microorganisms into the formation and allowing the microorganisms to acclimate to the formation environment.

3. The process of claim 1 further comprises, after act (e), the additional act of introducing one or more microorganisms into the formation and allowing the microorganisms to acclimate to the formation environment and repeating acts (a) through (e).

4. The process of claim 1 wherein act (c) comprises (1) identifying a genetic characterization of at least one hydrocarbon-degrading microorganism of the consortia and comparing said characterization with at least one known genetic characterization derived from a known microorganism having one or more known physiological and ecological characteristics and (2) identifying a genetic characterization of at least one methanogenic microorganism of the consortia and comparing said characterization with at least one known genetic characterization derived from a known microorganism having one or more known ecological characteristics.

5. The process of claim 1 wherein the analysis of one or more components of the formation comprises obtaining and analyzing one or more samples of material from the formation, the material comprising at least one of formation water, formation hydrocarbons, or formation solids.

6. The process of claim 5 wherein the analysis of the fluid samples comprises identifying components of the fluids and state values of the fluids in the formation.

7. The process of claim 1 wherein characteristics of the microorganisms of act (c) is determined using a DNA analysis technique.

8. The process of claim 1 wherein identification of methanogenic microorganisms is performed using polymerase chain reaction technology amplifying a portion of methyl reductase genes.

9. The process of claim 1 further comprises determining an ecological environment that promotes in situ degradation of formation hydrocarbons by at least one microorganism of the consortia and promotes generation of methane by at least one methanogenic microorganism of the consortia by growing the identified microorganisms of act (b) in one or more test media containing formation fluid at a selected temperature, pressure and salinity using a multiplicity of growth stimulants.

10. The process of claim 9 wherein the growth stimulants used in the test media comprise at least one of (1) nutrients, (2) electron acceptors, (3) trace elements, (4) buffers, (5) vitamins, (6) catalysts, or (7) combinations thereof.

11. The process of claim 1 further comprises modifying the formation environment by injecting into the formation an aqueous solution containing a microbial stimulant.

12. The process of claim 11 wherein the microbial stimulant comprises at least one of (1) nutrients, (2) electron acceptors, (3) trace elements, (4) buffers, (5) vitamins, (6) catalysts, or (7) combinations thereof.

13. The process of claim 11 wherein the microbial stimulant comprises one or more compounds containing at least one of nitrogen or phosphorus.

14. The process of claim 11 wherein the microbial stimulant is one or more electron acceptors comprising at least one of nitrate, CO₂, mineral oxides, humic acid, quinone compounds, sulfate, Fe³⁺, oxygen, or combinations thereof.

15. The process of claim 11 wherein the aqueous solution injected into the formation is fluid flood of a hydrocarbon recovery operation.

16. The process of claim 15 further comprises regulating the injection rate of the fluid flood into the formation in which the formation comprises at least two zones of fluid, each zone of fluid having different fluid densities to create multiple environmental interfaces between at least one formation zone in which hydrocarbon degradation predominates and at least one other formation zone in which methanogenesis predominates.

17. The process of claim 15 further comprises alternating injection of two or more microbial stimulants into the formation.

18. The process of claim 1 further comprising, after modifying the formation environment, shutting in the formation.

19. The process of claim 1 further comprises modifying the formation environment by injecting into the formation an aqueous solution that modifies at least one of formation salinity or formation temperature.

20. The process of claim 1 wherein the modification of the formation environment comprises injecting into the formation a material that changes the concentration of CO₂ in the formation.

21. The process of claim 1 wherein the modification of the formation environment comprises injecting into the formation a material that modifies the concentration of O₂ in the formation.

22. The process of claim 1 wherein the modification of the formation environment comprises injecting into the formation a material that changes the concentration of H₂ in the formation.

23. The process of claim 1 wherein the modification of the formation environment comprises stimulating growth of hydrocarbon-degrading microorganisms in the formation to convert the hydrocarbons to H₂ and CO₂.

24. The process of claim 1 wherein the modification of the formation environment comprises stimulating growth of hydrocarbon-degrading microorganisms in the formation to convert the hydrocarbons to at least one of alcohols, compounds containing amines, and acids.

25. The process of claim 1 wherein the modification of the formation environment comprises injecting into the formation at least one material that suppresses growth of microorganisms in the formation that compete with the methanogenic microorganisms, thereby promoting growth of methanogenic microorganisms.

26. The process of claim 1 further comprising the act of recovering from the formation methane produced by methanogenic microorganisms.

27. The process of claim 26 wherein the methane recovery is from a gas zone formed by the methane produced by the microorganisms.

28. The process of claim 1 wherein the methane is dissolved in fluid produced at a production well.

29. The process of claim 1 further comprising monitoring formation environment during modification of the formation environment of act (e).

30. The process of claim 1 wherein the hydrocarbon-bearing formation is at least one of peat, lignite, coal, oil shale, oil formation, or tar sands.

31. The process of claim 1 wherein, in addition to methane, the process converts formation hydrocarbons to other low molecular weight hydrocarbons.

32. The process of claim 1 wherein the one or more components of the formation comprises one or more fluids of the formation.

33. The process of claim 1 wherein the one or more components of the formation comprises one or more fluids of the formation and formation rock.

34. The process of claim 1 wherein the hydrocarbons being converted by the microbial action comprise contaminant-hydrocarbons.

36. The process of claim 31 further comprising the act of subjecting the formation to a methane recovery means.

37. The process of claim 31 further comprising after act (e) shutting in the formation to allow the microorganisms to convert the hydrocarbons to methane.

35. A process of stimulating the activity of microbial consortia in a hydrocarbon-bearing, subterranean formation to convert formation hydrocarbons to methane, comprising the acts of:

(a) analyzing fluid and rock of the formation to determine characteristics of the formation environment;

(b) detecting the presence of microbial consortia in at least one formation sample;

(c) determining a genetic characterization of at least one hydrocarbon-degrading microorganism consortia, and comparing said genetic characterization with at least one known genetic characterization derived from a known hydrocarbon-degrading microorganism having one or more known physiological and ecological characteristics;

(d) determining a genetic characterization of at least one methanogenic microorganism, and comparing said genetic characterization with at least one known genetic characterization derived from a known methanogenic microorganism having one or more known physiological and ecological characteristics;

(e) using information obtained from acts (a), (c), and (d) for determining an ecological environment that promotes in situ microbial degradation of formation hydrocarbons and promotes microbial generation of methane by at least one methanogenic microorganism of the consortia; and

(f) modifying the formation environment based on the determinations of act (d) to stimulate and sustain microbial conversion of formation hydrocarbons to methane.