Flow analysis cell and layered sensor pertaining thereto

• US 6,544,393 B1
• Filed: 08/31/2000
• Issued: 04/08/2003
• Est. Priority Date: 01/16/1998
• Status: Expired due to Fees

• Current Assignee: Trace Biotech AG
• Original Assignee: Trace Biotech AG
• Inventors: Künnecke, Wolfgang
• Primary Examiner(s): Tung, T.
• Assistant Examiner(s): NOGUEROLA, ALEXANDER STEPHAN
• Application Number:
  US09/600,142
• Time in Patent Office: 950 Days
• Field of Search: 204/403.01, 204/403.14, 204/409, 204/416, 204/433, 422/82.01, 422/82.03, 422/82.02, 422/82.05
• US Class Current: 204/409
• CPC Class Codes: G01N 27/403 Cells and electrode assemblies

Abstract

The analysis flow cell provided with a thin-film or thick-film sensor is designed so that the input and outlet for the fluid to be investigated are on opposite sides of the sensor layer and so that there is at least one passage (24) transverse to the sensor film, which can be in the sensor film or beside it in a layer of carrier. The sensor can be a biosensor, in which a thin layer of platinum, gold, or graphite is coated with a biosensor material.

Claims

1. Analytical flow cell, comprising:

a planar sensor;

a cell having a cell volume in contact with the sensor, said cell volume having an inlet and an outlet to allow flow of a fluid medium to be analyzed; and

wherein the sensor has at least one defined passage that is of constant diameter transverse to the sensor for a flow medium to be analyzed, with said inlet and said output on opposite sides of the sensor.

2. The analytical flow cell according to claim 1, wherein the cell volume is made of approximately conical shape with the base of the conical shape adjacent to a sensitive area of the sensor.

3. The analytical flow cell according to claim 1 or 2, further comprising a collecting volume on an outlet side of the cell volume directly behind the sensor.

4. The analytical flow cell according to claim 3, wherein said input is comprised of multiple inlets and said output is comprised of multiple outlets.

5. The analytical flow cell according to claim 3, further comprising a holder which holds connectors for input and removal of the flow medium to be analyzed and for taking off signals obtained with the sensor to a detector unit, wherein the cell is placed within the holder, and the holder having

means to obtain signals from the sensor and

means for separably mounting the sensor in a position in contact with the cell volume.

6. The analytical flow cell according to claim 5, wherein the holder is made up of at least two parts separably connected together, between which the sensor is placed.

7. The analytical flow cell according to claim 6, wherein the holder is made up of said at least two parts that can be unfolded.

8. The analytical flow cell according to claim 5, wherein the holder is of metal or plastic, and wherein the cell has a geometry that is made so that it is integrated into the holder or into a replaceable fluidic part in the holder.

9. An analytical flowcell comprising a film sensor that is a biosensor or an ion-selective electrode and has at least one defined passage that is of constant diameter so that a fluid medium to be analyzed can flow through it transverse to the film.

10. The analytical flowcell according to claim 9, wherein the sensor is an ion-selective electrode.

11. The analytical flowcell according to claim 9, wherein the sensor is a biosensor or an ion-selective electrode comprising a thin film fixed onto a carrier.

12. The analytical flowcell according to claim 11, wherein the at least one defined passage is formed in the thin film or immediately adjacent to the thin film in the carrier.

13. The analytical flowcell according to claim 9, wherein the at least one defined passage has a circular cross section.

14. The analytical flowcell according to claim 9, wherein the sensor comprises a multielectrode pattern printed on a carrier with at least one electrode having at least one defined passage of constant diameter transverse to the film.

15. The analytical flowcell according to claim 14 or 11, wherein a sensitive film of the sensor applied to a carrier is in the immediate vicinity of the at least one defined passage, adjacent to the at least one defined passage, projecting into the at least one defined passage, or passing through the at least one defined passage.

16. The analytical flowcell according to claim 15, wherein the sensor is constructed in a manner which allows measuring glucose, alcohol, lactate, glutamine, glutamate, sulfite, or hydrogen peroxide.