Multilayered microfluidic DNA analysis system and method
First Claim
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1. A DNA analysis system comprising:
- a sample inlet port;
a cell lysis chamber in fluid communication with said sample inlet port;
a DNA separation chamber in fluid communication with said cell lysis chamber;
a DNA amplification chamber in fluid communication with said DNA separation chamber, said DNA amplification chamber being defined by a substantially monolithic structure, said substantially monolithic structure being formed from a plurality of green-sheet layers sintered together, said green-sheet layers including particles selected from the group consisting of ceramic particles, glass particles, and glass-ceramic particles; and
a DNA detection system in fluid communication with said DNA amplification chamber.
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Abstract
A multilayered microfluidic DNA analysis system includes a cell lysis chamber, a DNA separation chamber, a DNA amplification chamber, and a DNA detection system. The multilayered microfluidic DNA analysis system is provided as a substantially monolithic structure formed from a plurality of green-sheet layers sintered together. The substantially monolithic structure has defined therein a means for heating the DNA amplification chamber and a means for cooling the DNA amplification chamber. The means for heating and means for cooling operate to cycle the temperature of the DNA amplification chamber as required for performing a DNA amplification process, such as PCR.
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Citations
30 Claims
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1. A DNA analysis system comprising:
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a sample inlet port;
a cell lysis chamber in fluid communication with said sample inlet port;
a DNA separation chamber in fluid communication with said cell lysis chamber;
a DNA amplification chamber in fluid communication with said DNA separation chamber, said DNA amplification chamber being defined by a substantially monolithic structure, said substantially monolithic structure being formed from a plurality of green-sheet layers sintered together, said green-sheet layers including particles selected from the group consisting of ceramic particles, glass particles, and glass-ceramic particles; and
a DNA detection system in fluid communication with said DNA amplification chamber. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15)
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16. A method for performing DNA analysis, said method comprising:
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placing in a fluidic sample containing cells in a cell lysis chamber;
lysing said cells in said cell lysis chamber to release cell contents, said cell contents including sample DNA;
passing said cell contents to a DNA separation chamber;
adsorbing said sample DNA onto a plurality of micro-beads in said DNA separation chamber;
eluting said sample DNA from said micro-beads;
passing said sample DNA to a DNA amplification chamber within a substantially monolithic structure formed from a plurality of green sheet layers sintered together;
amplifying said sample DNA in said DNA amplification chamber to produce amplified DNA; and
detecting said amplified DNA. - View Dependent Claims (17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30)
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Specification